摘要
研究了一种以活性／可控自由基聚合（可逆加成一断裂链转移自由基聚合，reversible addition-fragmentation chain transfer radical polymerization，RAFT polymerization）制备分子印迹微球的方法。结合沉淀聚合法，以二硫代苯甲酸异丙苯酯（cumyl dithiobenzoate，CDB）为可逆加成一断裂链转移剂，恩诺沙星为印迹化合物，甲基丙烯酸（MAA）和乙二醇二甲基丙烯酸酯（EDMA）分别为功能单体和交联剂，偶氮二异丁腈（AIBN）为引发剂，乙腈为分散剂，考察了温度、单体浓度等反应条件对微球制备的影响。通过改变AIBN和CDB的比例，可以达到控制分子印迹微球粒径和分散性的目的。扫描电镜结果显示，以RAFT聚合法得到的分子印迹微球粒径可达2．0～2．2μm，并有良好的分散性。平衡吸附实验结果说明印迹聚合物微球对印迹化合物的结合能力优于非印迹聚合物。分子印迹微球对于恩诺沙星及其结构类似物洛美沙星的饱和吸附量分别为37．3和19．5μmol·g^-1，证明分子印迹微球有很好的识别能力。
The method of using one living/controlled radical polymerization（ reversible addition-fragmentation chain transfer radical polymerization, RAFT ploymerization） for the sythesis of molecularly imprinted microsphere was developed. Combined with the precipitation polymerization, the MIP microspheres were prepared using acetonitrile as the dispersant, enrofloxacin as the template molecule, methyacrylic acid （MAA） as the functional monomer, ethylene dimethacrylate（EDMA） as the crosslinker, cumyl dithiobenzoate （CDB） as the RAFT agent and azobisisobutyronitrile（AIBN） as the initiation agent. The effects of the reaction conditions including the temperature and monomer concentration on the preparation were studied. The diameter and dispersity of MIP micropheres could be controlled through the ratio of AIBN and CDB. The results of scanning electron microscopic images showed that better particle size distribution of MIP microspheres with a diameter range of 2.0 - 2. 2 μm was obtained. The equilibrium adsorption experiment showed that the binding ability of MIP for the template molecule was superior to that of non-imprinted polymer. The amount of saturation adsorption for enrofloxacin and lomefloxacin were 37.3 and 19.5 μmol·g^-l, respectively, indicating that the MIP microspheres could well recognize the template molecule.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第10期1025-1030,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(20575030)
作者简介
张毅（1978-），男，天津人，讲师，硕士研究生通讯作者：
董襄朝，Tel：022-23504694，E-mail：xedong@nankai．edu．cn
