摘要
目的探讨乙肝病毒感染免疫学标志物常见模式与HBV DNA含量检测之间的关系。方法收集225例辽宁省乙型肝炎病毒感染者及20例健康对照者血清,采用ELISA方法进行乙型肝炎病毒免疫学标志物检测,并采用荧光定量PCR方法检测其HBV DNA含量。结果各组之间DNA含量有差异(P<0.001)。大三阳组患者血清HBV DNA阳性检出率明显高于除HBsAg(+)、HBeAb(+)、HBcAb-IgM(+)组(P=0.232)以外的其他各组(P<0.001)。HBeAg阳性和HBV DNA阳性有较高的相关性(P<0.001)。各组之间ALT含量无差异,而ALP、GGT均存在差异(P<0.05)。结论辽宁地区各模式分组中大三阳组患者HBV DNA的阳性检出率及HBV DNA的平均含量最高,HBeAg检测和DNA定量检测两者呈正相关,HBV DNA含量与肝功能的损坏程度可能相关。
Objective To investigate the correlation between immunological markers in common mode and HBV DNA levels within hepatitis B virus infection. Methods Collecting 225 serum of hepatitis B virus infectors and 20 healthy control, immunological markers of hepatitis B virus were detected by ELISA method and HBV DNA level were detected by fluorescence quantitative PCR（FQ- PCR）. Results There were differences of HBV DNA among each group（ P 〈 0.001 ）. And the detection rate of HBV DNA in the group of HBsAg（ ＋ ）、HBeAg（ ＋ ）、HBcAb（ ＋ ）was obviously higher than other groups except the group of HBsAg（ ＋ ）、HBeAb （ ＋ ） ,HI？cAb - IgM（ ＋ ） （ P 〈 0. 001 ）. The detection rate between HBeAg and HBV DNA have obvious dependability（ P 〈 0.001 ）. The content of ALT have no difference among each group, while inversely both ALP and GGT have（ P 〈 0.05）. Conclusion The detection rate of HBV DNA and the average content of HBV DNA in HBsAg（ ＋ ） ,HBeAg（ ＋ ） ,HBcAb（ ＋ ）group is higher than other groups. The detection rate between HBeAg and HBV DNA have positive correlation. There are relationships between HBV DNA and the deterioration degree of liver function, approximately.
出处
《中国实验诊断学》
2008年第10期1285-1287,共3页
Chinese Journal of Laboratory Diagnosis
