摘要
背景：羟基磷灰石降解缓慢、脆性大，而丝素蛋白机械强度差，将两者复合，可能克服单个材料的缺点。目的：测试不同规格丝素蛋白／羟基磷灰石（silk fibroin／hydroxyapatite，SF／HA）复合材料的吸水率和体内降解速率，为进一步的成骨实验提供依据。设计、时间及地点：随机对照的体内及体外实验，于2006-09／2007-06在苏州大学附属第一医院骨科研究所实验室完成。材料：4种规格SF／HA—1、SF／HA-2、SF／HA-3、SF／HA-4及普通羟基磷灰石，各材料的差别主要是丝素蛋白与羟基磷灰石组成比、致孔剂量及添加剂量不同。方法：测定4种SF／HA复合材料和羟基磷灰石在0．5，2，6，12及24h不同时间点的吸水率。将4种SF／HA复合材料植入SD大鼠背部皮下，进行体内降解观察，以羟基磷灰石为对照组。主要观察指标：各材料的吸水率；大体及组织学检查观察材料植入SD大鼠背部后不同时间点的降解情况。结果：各材料吸水率趋势为SF／HA-3和SF/HA-4〉SF/HA-2及SF／HA-1〉羟基磷灰石；同一材料吸水率随时间延长变化不明显。羟基磷灰石、SF／HA-1及SF／HA-2降解十分缓慢或基本不能降解：SF／HA-3于20-24周降解完毕；SF／HA-4于12～16周降解完毕；各种材料周围组织未见明显变性坏死等。结论：丝素蛋白能改善羟基磷灰石的理化性能，并可调控SF／HA的降解速率，SF／HA复合材料具有良好的组织相容性，有望成为一种新的骨修复替代材料。
BACKGROUND： Hydroxyapatite exhibits slow degradation and great fragility, while silk fibroin is weak in the mechanical strength, so the combination of the two will benefit to overcome their own shortcomings. OBJECTIVE： To test the water absorption rate and degradation rate in vivo of silk fibroin/hydroxyapatite （SF/HA） composite, so as to provide evidences for further study on the bone formation of SF/HA. DESIGN, TIME AND SETTING： A randomized and controlled experimental study was carried out in the Laboratory of Orthopaedics Department, the First Affiliated Hospital of Soochow University from September 2006 to Jun 2007. MATERIALS： Four kinds of SF/HA-1, SF/HA-2, SF/HA-3, SF/HA-4 and common HA. They varied each other with the component ratio of SK/HA, pore-forming dosage and additives＇ dosage. METHODS： The water absorption rate of 4 types of SF/HA composites and HA were measured at 0.5, 2, 6, 12 and 24 hours after these materials soaked in deionized water. Four SF/HA composites were implanted into SD rats＇ back subcutaneously to observe their degradation rate in vivo. MAIN OUTCOME MEASURES： The water absorption rate of all materials was measured; and the degradation of these materials were evaluated by gross observation and histological examination in SD rats. RESULTS： Water absorption rates in 4 kinds of SF/HA composites were as follows： SF/HA-3 and SF/HA-4〉SF/HA-2 and SF/HA-1〉HA. HA, SF/HA-1 and SF/HA-2 had little degradation. SF/HA-3 had complete degradation in 20-24 weeks after operation, while SF/HA-4 had complete degradation in 12-16 weeks. Tissue surrounding all materials had no apomorphosis or necrosis. CONCLUSION： SF can improve physical and chemical properties of HA, and change the bio-degradation rate of SF/HA. The composite of SF/HA has a good histocompatibility, and may be suitable for bone tissue engineering scaffold.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第36期7057-7060,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
作者简介
王根林，男，1972年生，安徽省怀宁人，汉族，2008年苏州大学毕业，博士，医师，主要从事脊柱外科、创伤骨科和人工骨研究。wglpaper@126．com.
通讯作者：杨惠林，苏州大学附属第一医院骨科，江苏省苏州市215006 wglpaper@126．com.
