摘要
研究了口服不同剂量海藻硫酸多糖、壳聚糖对刺参(Apostichopus japonicus)酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LSZ)、超氧化物歧化酶(SOD)活性的影响。在基础饲料中分别添加0.25%、0.5%、1.0%海藻硫酸多糖(质量百分比);0.5%、1.0%、2.0%壳聚糖,以基础饲料饲喂组为对照,饲喂7d。于投喂后第2天、5天、9天和15天检测ACP、AKP、LSZ与SOD活性。结果显示,投喂后15d内,各实验组ACP、AKP活性均随时间和剂量增加持续升高,与对照组具有显著性差异(P<0.05),其中添加1.0%海藻硫酸多糖与2.0%壳聚糖效果最佳,1.0%海藻硫酸多糖组的ACP、AKP酶活最高,分别是对照组的3倍、2.3倍,2.0%壳聚糖添加组的ACP、AKP活性分别是对照组的3.9倍、4.4倍;LSZ活性则随时间延长先升高后降低,最高时与对照组具有显著性差异(P<0.05),且LSZ活性与免疫多糖添加量不成正比关系,0.5%海藻硫酸多糖与1.0%壳聚糖组效果最佳,酶活最高分别是对照组的1.4倍、3.3倍;SOD活性均在第2天显著升高,短暂的升高后逐渐降低趋于对照,1.0%海藻硫酸多糖、2.0%壳聚糖组SOD酶活最高,分别是对照组的1.7倍、2.1倍。研究结果表明,海藻硫酸多糖和壳聚糖可作为刺参免疫增强剂使用,建议其适宜添加量分别为1.0%海藻硫酸多糖、2.0%壳聚糖。
The effects of seaweed-sulfated polysaccharides and chitosan on the activities of acid phosphatase （ACP）, alkaline phosphatase （AKP）, lysozyme （LSZ） and superoxide dismutase （SOD） of sea cucumber, Apostichopus japonicus were studied by supplying various seaweed-sulfated polysaccharides or chitosan levels in diets. The experimental dietary levels of seaweed-sulfated polysaccharides were 0.25% （m/m）, 0.5% and 1.0%, and those of chitosan were 0.5%, 1.0% and 2.0%, respectively. Group fed with basic feed was set as control. On the 2nd, 5th, 9th and 15th day after feeding for 7 days, the activities of ACP, AKP, LSZ and SOD in sea cucumbers were determined. The results indicated that the activities of ACP and AKP both sustainably increased and had significant differences compared with the control （P〈0.05）. The effects of 1.0% seaweed- sulfated polysaccharides and 2.0% chitosan on the activities of ACP and AKP were the best. The highest activities of ACP were 3 and 3.9 times of that of control, and the highest AKP activities were 2.3 and 4.4 times of that of control, respectively. The activities of LSZ increased firstly after immunopolysaccharide feeding, with the tip-top significantly different compared with the control （P〈0.05）. However, the activities of LSZ were not proportionally related to the additional dosage of immunopolysaccharide. The effects of 0.5% seaweed-sulfated polysaccharides and 1.0% chitosan on the activities of LSZ were the best. The highest LSZ activities were 1.4 and 3.3 times of control respectively. The activities of SOD were all significantly enhanced on the 2nd day after feeding in all treatment groups, and then decreased. The effects of 1.0% seaweed-sulfated polysaccharides and 2.0% chitosan on the activities of SOD were the best. The highest SOD activities were 1.7 and 2.1 times of that of control, respectively. In conclusion, seaweed-sulfated polysaccharides and chitosan can regulate the antioxidant enzymes and hydrolytic enzymes, and can improve the non-specific immunity of sea cucumber. Taking both economy and efficiency into account, it is suggested that the feasible dosage should be 1.0% （m/m） seaweedsulfated polysaccharides and 2.0% （m/m） chitosan.
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第5期787-793,共7页
Journal of Fishery Sciences of China
基金
国家自然科学基金项目(30000129).
关键词
免疫多糖
剌参
酸性磷酸酶
碱性磷酸酶
溶菌酶
超氧化物歧化酶
immunopolysaccharide： Apostichopusjaponicus： acid phosphatase
alkaline phosphatase： lysozyme：superoxide dismutase
作者简介
刘云（1969-），女，博士，副教授，主要从事海洋动物免疫研究．Tel：0532—82032913：E-mail：liuyun@ouc．edu．cn.
