摘要
用AFLP分子标记技术对芸香科Rutaceae柑橘亚科Aurantioideae真正柑橘果树类(the True Citrus Fruit Trees Group)6属植物的59个生物类型以及九里香Murraya exotica L.、蚝壳刺Severinia buxifolia Tenore和酒饼簕Atalantia buxifolia Oliv.进行了分析。从64对引物组合中筛选出15对分辨率好的引物对全部材料进行了扩增,共获得312条带,其中多态型带305条,多态位点达97.8%。利用PAUP软件构建了全部材料基于AFLP数据的UPGMA距离树。结果表明,AFLP证据支持基于形态特征建立的真正柑橘果树群以及群内各属的划分,只是金柑属Fortunella Swingle作为一个独立的分支嵌于柑橘属CitrusL.内。对于柑橘属,我们的分子证据支持Swingle关于亚属的划分以及Scora等关于C.medica、C.grandis和C.reticulata是真正柑橘亚属subgen.Eucitrus三个基本种的观点。AFLP分子证据显示宜昌橙C.ichangensis Swingle位于真正柑橘亚属中,与香橙C.junos Sieb.exTanaka的亲缘关系最近,而与大翼橙亚属subgen.Papeda中马蜂柑C.hystrix DC.相隔甚远。另外,莽山野橘C.mangshanensis S.W.He & G.F.Liu在系统树上居于宽皮柑橘类的基部。最后,对真正柑橘果树群6属植物间以及柑橘属各主要类型(种)的进化关系进行了详细的讨论。
Fifty-nine genotypes representing six genera of the True Citrus Fruit Trees Group （Aurantioideae, Rutaceae） were analyzed using AFLP technique to study their taxonomic and phylogenetic relationships. Fifteen primer combinations （out of 64 screened） were selected based on the polymorphism and quality of the bands produced by the primer and used in the present study. A total of 312 bands were obtained, of which 305 （97.8%）, were polymorphic. The UPGMA tree of all genotypes was constructed based on the AFLP data using PAUP＊ beta version 4.0b8 software. Our AFLP molecular tree clearly confirms that the True Citrus Fruit Trees Group is monophyletic and supports the division of the group into genera mainly based on morphological characters, except that the genus Fortunella was nested within the genus Citrus cluster （as a monophyletic sub-branch）. The subdivision of the genus Citrus into subgen. Papeda and subgen. Eucitrus as suggested by W. T. Swingle based on morphological characters and the point of view that C. medica, C. grandis and C. reticulata are the three basic species of the subgen. Eucitrus were also supported by our molecular data. In addition, contrary to the expectation based on morphological data, however, our molecular data demonstrated that C. ichangensis is more closely related to C. junos of the subgen. Eucitrus than to the C. hystrix of the subgen. Papeda. Furthermore, our study provided the first evidence that C. mangshanensis is basal to all the loose-skin citrus types. Finally, the taxonomic and phylogenetic relationships among the six genera and the important genotypes of the genus Citrus were discussed in detail.
出处
《植物分类学报》
CSCD
北大核心
2008年第5期682-691,共10页
Acta Phytotaxonomica Sinica
基金
国家自然科学基金资助项目(No.30671450)资助
关键词
AFLP
进化
谱系关系
真正柑橘果树类
AFLP, evolution, molecular phylogeny, the True Citrus Fruit Trees Group.
作者简介
通讯作者（Author for correspondence E-mail：zqzhoubj@yahoo.com；Tel：86-23—68250029）。
