摘要
目的:研究黄芪对锰致多巴胺能神经元PC12细胞凋亡的拮抗作用。方法:体外染锰培养多巴胺能神经元PC12细胞,MTT比色法观察染锰PC12细胞的增殖情况;流式细胞仪(FMC)和原位缺口末端标记法(TUNEL)检测细胞凋亡状况及黄芪的拮抗作用。结果:锰对PC12细胞的生长有明显的抑制作用,并呈剂量-反应关系;FMC和TUNEL检测结果均显示锰能诱导PC12细胞凋亡,并呈浓度依赖性,而加入黄芪后,各组凋亡率明显降低。结论:锰能够诱导PC12细胞凋亡,黄芪对锰诱导PC12细胞的凋亡有一定的拮抗作用。
Objective： To study the antagonistic action of astragalus on dopaminergic neuron PC12 cells apoptosis induced by manganese. Methods：Using PC12 cells as the model of dopaminergic neuron was incubated in culture media with 200,400,800 umol/L manganese chloride （MnCl2） for 36 hours, MTT colorimetry test were performed to exam cell growth and livingness of PC12 ceils invarious dose of manganese;Manganese induced apoptosis and the Antagonistic action of astragalus in PC12 cells were observed by FMC and TUNEL. Results： MTT results found that manganese might suppress the proliferation of PC12 cells in dose-dependent manner and induce PC12 cells apoptosis, and apoptosis of PC12 cells treated with astragalus had decreased. Conclusions： Apoptosis of PC12 cells can induced by manganese, whereas astragalus has the antagonism to manganese.
出处
《中国冶金工业医学杂志》
2008年第5期527-528,共2页
Chinese Medical Journal of Metallurgical industry
关键词
锰
黄芪
PC12细胞
凋亡
拮抗作用
Manganese
Astragalus
PC 12 cells
Apoptosis
Antagonism
