摘要
目的建立兔的上胸段硬膜外阻滞(HTEB)模型和蛛网膜下腔出血(SAH)模型,探讨HTEB对SAH后脑血管痉挛(CVS)的预防作用。方法用切开置管法制备HTEB模型的新西兰兔30只,随机分为3组(n=10):正常组、对照组(单纯SAH)和实验组(HTEB+SAH)。采用枕大池穿刺一次注血法(0.5ml/kg)制成兔SAH模型。1d后用经颅多普勒检测兔基底动脉血流速度(Vm)。处死后取基底动脉,光镜下观察其形态学变化。结果与正常组相比,对照组和实验组的基底动脉Vm均明显升高(P<0.05或<0.01);但实验组的基底动脉Vm则明显低于对照组(P<0.05)。在光镜下,对照组基底动脉管壁收缩、管腔变窄,实验组变化程度轻于对照组。结论HTEB可以减轻兔SAH后急性CVS的程度。
Objective To investigate the preventive effects of high thoracic epidural block （HTEB） on acute cerebral vasospasm （CVS） following subarachnoid hemorrhage （SAH） in rabbits. Methods The HTEB model at T6-7 epidural space was established in 30 rabbits, who were divided into 3 groups of 10 rabbits each, i. e. the normal group, SAH group and HTEB group. CVS was developed by injection of non-anticoagulant autologous arterial blood （0.5 ml/kg） into the cisterna magna in SAH and HTEB groups where injection of the autologous arterial blood into the cistern magna was performed 30 minutes after HTEB. Transcranial Doppler ultrasonography was used to detect the basilar artery mean blood flow velocity （Vm） 1 day after SAH. The changes in pathohistology and calibre of the basilar artery were observed under a light Microscope. Results The Vm of the basilar artery in SAH group increased significantly compared to the normal group （P〈0.01）. The Vm in HTEB group was significantly slower than that in SAH group （P〈0.05）. Pathological observation showed that the lumina of the basilar arteries became more narrow in SAH group and the pathologic changes in the basilar artery walls was more serious than those in HTEB group. Conclusion HTEB used before SAH has the preventive effects on acute CVS in rabbits with SAH.
出处
《中国临床神经外科杂志》
2008年第9期542-545,共4页
Chinese Journal of Clinical Neurosurgery
关键词
上胸段硬膜外阻滞
脑血管痉挛
基底动脉
兔
High thoracic epidural block
Cerebral vasospasm
Basilar arteries
Rabbit
作者简介
现在厦门市第一医院麻醉科（福建厦门，361004）
通讯作者
