摘要
以南方红豆杉(Taxes chinensisvar.mairei Cheng et L.K.Fu)的幼叶、幼嫩茎段、花蕾为外植体进行组织培养育苗试验,结果表明:①外植体诱导愈伤组织的容易程度为幼嫩茎段>幼叶>花蕾;②诱导南方红豆杉产生愈伤组织的最佳培养基配方是:MS+6-BA 0.5 mg.L-1+2,4-D 1.0 mg.L-1;③BA、ZT和KT均能诱导愈伤组织分化不定芽,其中以0.1 mg.L-1ZT效果最佳,诱导率达58%;④生根培养阶段,以附加NAA1.0 mg.L-1+0.1%活性碳的1/2 MS培养基效果最好,生根率达96%;⑤试管苗移栽在河沙+泥炭的基质上,成活率可达95%。
In this experiment, young leaves, tender shoots and blossom buds of Tazus chinensis var. mairei were used to induce the callus. The result showed that： （1） for inducing the callus, the segments of tender shoots were the easiest, secondly were the leaves, finally were the blossom bud; （2） the optimum medium for inducing callus was： MS ＋ 6-BA 0.5 mg·L^-1 ＋ 2, 4-D 1.0 mg·L^-1 ; （3） BA, ZT and KT all could induce the adventitious buds from callus, among which, KT with the concentration of 0.1 mg·L^-1 was most effective, induced rate accounting for 58% ; （4）for inducing the roots, with the additional NAA 1.0 mg· L^-1 ＋ activated charcoal 0.1% into the 1/2 MS, the rooting rate reached 96 % ; （5） in the river sand mixed with turves, the survival rate of the tube seedlings reached the amount of 95 %.
出处
《福建林业科技》
北大核心
2008年第3期102-105,共4页
Journal of Fujian Forestry Science and Technology
基金
韶关学院蔬菜学重点学科资金(2005)
关键词
南方红豆杉
外植体
愈伤组织
诱导
组织培养
Taxus chinensis var. mairei Cheng et L. K. Fu
explant
callus
induction
tissue culture
作者简介
叶添谋（1972-）。男。广东汕尾人。韶关学院讲师。在职博士研究生。从事植物组织培养、天然产物工程研究。
