摘要
目的探讨海生素（HSS）抗白血病作用及其机制。方法分别以不同质量浓度（0．1—1000．0μg/ml）HSS处理白血病耐药细胞K562／ADM，采用MTT法测定细胞增殖情况，免疫细胞化学法检测凋亡相关蛋白Bcl-2、Bax表达，流式细胞仪（FCM）测定凋亡率，Western blot法检测caspase-3表达。结果HSS在较高质量浓度时可抑制K562／ADM细胞生长，且具有时效和量效性；经HSS处理的K562／ADM细胞呈现凋亡特有的亚G1峰，凋亡比率随HSS质量浓度和时间延长而增高；HSS处理后，K562／ADM细胞中Bcl-2呈阴性表达，Bax呈强阳性表达；caspase-3表达上调。结论HSS在体外可明显抑制K562／ADM细胞增殖；可能通过下调Bcl-2表达、上调Bax及caspase-3表达诱导K562／ADM细胞凋亡。
Objective To investigate the effect and mechanism of Haishengsu（HSS） on leukemia. Methods K562/ ADM cells of leukemia were treated with HSS of different concentration. And the survival rates of K562/ADM cells was dectcted by MTT assay, the expression of Bcl-2 and Bax was dectcted by immunocytochemistry, the rate of apoptosis was dectcted by flow cytometer, the expression of caspase-3 protein was dected by western bolt. Results The profileration and expression of Bcl-2 of K56/ADM cells decreased, the apoptosis rate and expression of Bax increased after treated with HSS. Conclusion Hss can significantly inhibit the proliferation and induce apoptosis of K562/ADM cells.
出处
《山东医药》
CAS
北大核心
2008年第23期21-23,共3页
Shandong Medical Journal
基金
山东省科学技术发展计划资助课题项目(2007GG20002011)
