摘要
为验证鸡粪好氧发酵过程同程硝化-反硝化细菌的存在，全程进行了高效同程硝化-反硝化菌株的分离、筛选，并对筛选的高效菌株进行鉴定和生物学特性研究。筛选到1株高效同程硝化-反硝化细菌JY45—2，鉴定为假单胞菌Pseudomonas sp．，为兼性化能自养型菌株。JY45-2菌株生物学特性为：当有机物质量浓度1360mg／L、氨态氮质量浓度212mg／L、pH8、C／N为3．54和培养温度45℃时，氨态氮利用效率最高。JY45—2菌株的有机物耐受范围宽，有机物质量浓度为680～2040mg／L时，作用3d后的氨态氮利用率可达30％以上。在鸡粪好氧发酵过程中分离、筛选到1株高效同程硝化-反硝化细菌，证明了该固态发酵体系中存在同程硝化-反硝化细菌。
In order to verify the existence of simultaneous nitrifying-denitrifying in composting of chicken manure, high- effective simultaneous nitrifying-denitrifying bacteria were isolated and screened. The bacteria were then identified and their biological characteristics were studied. The results showed that a high-effective simultaneous nitrifying-denitrifying strain JY45-2, belonging to facultative autotroph, was screened and identified as Pseudomonas sp. Its utilization rate to ammonium nitrogen reached the maximum in following conditions： concentration of organic compounds in 1 360 mg/ L, concentration of ammonium nitrogen in 212 mg/L, pH 8, C/N 3.54, and culture temperature at 45℃. The strain had a wide range of tolerance to organic compounds, and its utilization rate to ammonium nitrogen was above 30% when concentration of organic compounds changed from 680 mg/L to 2 040 mg/L. The study verified the existence of simultaneous nitrifying -denitrifying in this composting system.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2008年第5期63-69,共7页
Journal of China Agricultural University
基金
国家自然科学基金资助项目(C020305)
关键词
鸡粪
发酵
硝化
假单胞菌
chicken manure
composting
nitrification
Pseudomonas sp
作者简介
曹立群，硕士研究生；
王立群，教授，通讯作者，主要从事微生物学研究，E-mail：wangliqun2@163．com
