摘要
目的:建立记录小鼠在体海马"前穿通纤维-齿状回"(PP-DG)神经通路长时程增强(LTP)的方法。方法:动物麻醉后固定于立体定位仪上,参照立体定位参数将刺激电极插入至前穿通纤维,记录电极插入至DG颗粒细胞层,而后进行LTP的诱发和记录。结果:对各种实验条件优化后,成功记录了Balb/c小鼠海马PP-DG通路LTP。应用该方法对快速老化模型小鼠(SAM)的快速老化亚系SAMP8和抗快速老化亚系SAMRl海马神经突触可塑性进行考察,结果表明在体海马LTP与脑片LTP和行为学实验结果相符。结论:成功建立了小鼠在体海马PP-DG通路LTP的记录方法,可用于整体动物神经突触可塑性的评价。
Aim： To establish the method of long-term potentiation（LTP） recording in hippocampus in anaesthetized mice in vivo. Methods： Mouse was anaesthetized and then placed in the stereotaxic apparatus.The recording electrode was located at the cell body layer of dentate granule cells and the stimulating electrode at the perforant path according to stereotaxic parameters.Then the LTP was evoked and recorded. Results： Alter optimizing of experimental factor, the LTP in PP-DG path in anaesthetized Balb/c mice was successfully recorded.The changes of synaptic plasticity were also observed in SAMP8 and SAMRI by using the optimized method. The results were coincident with the behavioral tests and LTP in hippocampal slices that we reported before. Conclusion： The method of LTP recording in vivo in hippocampus in anaesthetized mice was successfully established and could be used to evaluate the synaptic plasticity in vivo.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2008年第3期291-295,共5页
Chinese Journal of Applied Physiology
基金
国家重点基础研究发展规划项目(2004CB518907)
国家自然科学基金资助项目(30500659
30772562)
关键词
LTP
在体
小鼠
海马
学习记忆
LTP
in vivo
mice
hippocampus
learning and memory
作者简介
黄晏（1982－），男，重庆忠县人，博士在读，从事中药药理和神经药理学研究。
通讯作者，E—mail：zhouwx@nic．bmi．ac．cn
