摘要
对翅鳞伞菌丝体的胞内粗蛋白进行分离、纯化、筛选,获得了抗水稻纹枯病菌的翅鳞伞纯化菌丝体蛋白PSPB-2。通过荧光显微镜观察PSPB-2对人肺癌细胞株(A549)的影响,发现PSPB-2会引起A549出现典型的细胞凋亡现象。进一步研究发现PSPB-2有限制性核酸内切酶的活性。说明菌丝体蛋白PSPB-2具有抗真菌作用的同时又具有抗肿瘤作用。
Mycelium protein PSPB-2 was separated,purified and screened from the mycelium proteins of Pholiota squarrosa.PSPB-2 remarkably inhibited the human lung cancer cell line A549 through observation by fluorescence microscope.PSPB-2 had antifungal activity to A549.And the further research showed that PSPB-2 had inhibition to endonuclease.So mycelium protein PSPB-2 had the antitumor activity as well as antifungal activity.
出处
《江西农业学报》
CAS
2008年第8期99-101,103,共4页
Acta Agriculturae Jiangxi
基金
国家"863"(NO.2007AA10Z357)
关键词
翅鳞伞
抗肿瘤蛋白
抗真菌
核酸内切酶
Pholiota squarrosa
Antitumor protein
Antifungal
Endonuclease
作者简介
汪建敏（1982-），男，安徽绩溪人，硕士研究生，从事微生物活性物质研究。
通讯作者：陆兆新。
