摘要
在不同pH值的Britton—Robinson缓冲溶液中，某些酸性三苯甲烷染料与盐酸吡格列酮反应，形成离子缔合物，其最大褪色波长在558—616nm区间，最大吸收波长位于426—450nm区间，盐酸吡格列酮的浓度在3．10×10^-7～1．55×10^-5mol／L不同范围内遵守比耳定律．表观摩尔吸光系数分别在1．47×10^4～9．50×10^4L／（mol·cm）内，检测限分别在0．0332—0．257mg／L内，建立了测定盐酸吡格列酮的光度法。方法有较高的灵敏度和良好的选择性，用于药品、血样、尿样中盐酸吡格列酮测定，结果满意。
In Britton-Robinson buffer media with different pH values, some acidic triphenylmethane dyes can form ion-association complexes with pioglitazone hydrolchloride respectively. The maximum fading wavelength of complexes is between 558 nm and 616 nm, and the maximum absorption wavelength is between 426 nm and 450 nm. The concentration of pioglitazone hydrolchloride follows Beer＇s law for the tight absorption in a range of 3.1 × 10^-7 mol/L and 1.55 × 10^-5 mol/L, the apparent molar absorptivity is between 1.47 × 10^4 L/（mol · cm） and 9.50 × 10^4 L/（mol · cm）, and the detection limit is between 3.32 × 10^-5 g/L and 2.57 × 10^-4 g/L respectively. A sensitive spectrophotometric method is therefore developed for the determination of pioglitazone hydrolchloride. The method has a high sensitivity and a fairly good selectivity, and has been applied to the detemfination of pioglitazone hydrolchloride in urine samples, mixture samples and blood samples, with satisfactory results.
出处
《应用化学》
CAS
CSCD
北大核心
2008年第9期1017-1021,共5页
Chinese Journal of Applied Chemistry
基金
重庆市教委科研(KJ081301)
"重庆市高等学校优秀中青年骨干教师资助计划"[渝教人(2005)2号]
长江师范学院科研(07Jkg50)资助项目
关键词
溴甲酚绿
氯酚红
酚红
溴甲酚紫
盐酸吡格列酮
分光光度法
bromocresol green, chlorophenol red,phenol red, bromocresol purple, pioglitazone hydrolchloride, spectrophotometry
作者简介
通讯联系人：秦宗会，男，副教授；E-mail：qinzonghui654321@163．com；研究方向：光谱分析，药物分析.
