摘要
以洋桔梗的叶片、不定芽、茎段等外植体为试验材料研究自然光及NH4+对洋桔梗玻璃化的影响。结果表明,同一培养基配方洋桔梗正常不定芽诱导率在自然光下比培养室内提高30%左右;NH4+除去或减半对洋桔梗玻璃化防治无明显效果,反而使同一条件下洋桔梗叶片诱导总不定芽数及正常芽数有所降低;自然光对已经玻璃化的不定芽转化无明显效果。
Ef gated. On the sa creased 30% und halved NH4^＋ med fect of sunlight and NH4^＋ on vitrification of Enstoma grandiflorun in leaf／bud／stem was investi me medium er daylight ium and su and normal buds reduced on , the results showed that normal adventitious bud rate of Enstoma grandiflorun inthan that in the culture room. There was no significant effect of removed NH4^＋ or on vitriification of Enstoma grandiflorun, however, the total adventitious buds NH4^＋ removed or halved NH4^＋ media compared to perfect medium. Sunlight showed no obvious effect on vitrified adventitious buds transformation to normal ones
出处
《广西农业科学》
CSCD
2008年第4期519-522,共4页
Guangxi Agricultural Sciences
基金
广西农科院科技发展基金项目(2007010)
关键词
洋桔梗
自然光
NH4^+
玻璃化
Enstoma grandiflorun.
sunlight
NH4^＋
vitrification
作者简介
陈小凤（1975-），女，广西南宁人，硕士，主要从事组织培养及生物技术方面的研究。
通讯作者。
