摘要
对数生长后期海水绿藻Platymonas subcordiformis具有较高氢酶活性,分别采用培养基中无硫和添加解偶联剂CCCP的方法研究其光照产氢特征。结果表明:无硫连续光照期间,PSⅡ保持较高放氧活性,不能诱导氢酶表达进行光照产氢;厌氧暗诱导32h后,加入5、10、15和20μmol/L的解偶联剂CCCP后光照,PSⅡ光化学活性和光合放氧能力明显被抑制,藻液体系保持厌氧状态,能够持续光照产氢12h以上,光照产氢量分别为21.8、23.0、22.5和16.0μmol/mg Ch1;合适浓度的CCCP能够促进绿藻直接光照产氢,但光照产氢量和产氢速率较低。
The hydrogenase activity of the marine green algae Platymonas subcordiformis at the late growth phase was the highest. The characterizations of photobiological hydrogen production for algae by sulfur element deprived from medium and treated with uncoupler CCCP were investigated. The sulfur element couldn＇t effectively regulate PSII photochemical activity, therefore it couldn＇t produce any hydrogen by sulfur-deprived during direct light illumination because hydrogenase couldn＇t expression. When algae treated with 5, 10, 15 and 20 μmol/L CCCP after 32 hours of dark anaerobic incubation of 32 hours, the PSII photochemical activity was obviously repressed, and the sealed system hold anaerobic condition, which could photoproduce 21.8, 23.0, 22.5 and 16.0 μmol/mg Chl, respectively. Besides, appropriate concentration CCCP could regulate algae hydrogen production under direct light illumination, but the quantity and rate of hydrogen production were lower than that of the former.
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2008年第8期944-949,共6页
Acta Energiae Solaris Sinica
基金
国家高技术研究发展(973)计划(2003CB214506)
大连民族学院博士启动基金(20076106)
关键词
海水绿藻
氢气
无硫
CCCP
光化学活性
marine green algae
hydrogen
sulfur-deprived
CCCP
photochemical activity
作者简介
通讯作者：冉春秋（1976-），男，博士、讲师，主要研究方向环境生物技术。rancq@dlnu．edu．cn
