摘要
建立高效液相色谱法测定腊肉中苯并[α]芘（简称BaP）的测定方法。通过先超声提取，后皂化的处理，采用迪马C18色谱柱（250mm×4．6mm，5μm）；乙腈-水为流动相；荧光检测器，激发波长为385nm；检测波长为405nm；苯并[a]芘在（0．005-1．0μg／mL）范围呈线性，其线性回归方程为：Y=1．8×10^7x＋11012；相关系数R=0．9992；苯并[a]芘在0．0035、0．0070、0．0105μg3个添加浓度水平上的回收率为（83．08％、88．67％、84．54％）。本法色谱条件易于掌握，测得数据重复性好，线性关系好，精密度和准确度均能满足分析要求。同时本文对贵阳市主要市售腊肉中苯并[α]芘（BaP）含量进行了测定，结果含量在1．04～4．16μg／kg之间，表明传统的油炸食品安全性使人的健康受到不同程度的影响。
High performance liquid chromatography method was used to determin benzo（a） pyrene in preserved ham. By ultrasonic extraction and saponification, the working conditions of LC： separate column was Diamonsil C18（4.6mm × 250mm, 5μm）, mobile phase was CH3CN-H2O,the excitation wavelength is 385nm, by fluorescence detector and detection wavelength is 405nm. The calibration curve was linear in the range of 0. 050 -1.0 μg/mL, Y = 1.8 × 10^7x ＋ 11012, R = 0. 9992; added to 0. 0035, 0. 0070 and 0. 0105μg, their recovery rate was 83.08% ,88.67% and 84.54%, respectively. The result show that the method has higher precision and accuracy in determination of benzo（a） pyrene . At the same time, the paper reported that content of benzo （a） pyrene preserved ham between 1.04 -4.16 μg/kg, which showed security of traditional preserved ham. had an impact on people＇ s health.
出处
《贵州师范大学学报（自然科学版）》
CAS
2008年第3期41-44,共4页
Journal of Guizhou Normal University：Natural Sciences
基金
贵州省科学技术基金黔科合J字[2007]2023
关键词
苯并[α]芘
腊肉
高效液相色谱
测定
benzo（α） pyrene
preserved ham
high performance liquid chromatography
determination
作者简介
曲明昕（1981-），女，汉族，硕士，研究方向：环境分析。
