摘要
采用组织病理学观察和RT-PCR检测对贵州省某鸡场一批230日龄疑似禽淋巴细胞性白血病的蛋种鸡群进行诊断。结果表明，该批发病蛋鸡群的眼观病变明显，肝脏、脾脏、肾脏、法氏囊和盲肠扁桃体肿大2～3倍；组织学检查显示肝、肾、法氏囊等组织中有大量大小一致的成淋巴细胞散在或呈团块状增生，挤压并破坏正常组织；选取针对禽白血病病毒群特异性抗原p27基因设计引物，经RT—PCR检测，可从发病蛋鸡肝和脾组织病料中扩增出与预期大小相一致（分子长404bp）的DNA片段，其中肝的特异性病原核酸的检出率达到90％，脾的特异性病原核酸的检出率达到50％。以上结果证实，该蛋鸡场存在禽淋巴细胞性白血病的感染，且通过PCR检测可得知肝脏的病原核酸检出率明显高于脾脏。
Cases suspected to be avian lymphoid leukosis from one batch 230 days breeding layer of one farm in Guizhou province were examined by histopathologic observation and RT - PCR technique. The results showed that this batch layers＇ gross anatomic lesions were significant, and liver, spleen, kidney, bursa and cecal tonsil were swelled by 2 - 3 times; histological inspection showed that, there were a lot uniform -sized lymphoblasts interspreaded or distributed by massive hyperplasia in liver, kidney and cecal tonsil, which extruded and destructed the normal tissues;then selected the p27 gene segment of group - specific antigen of avian leukosis virus and amplified out the expected length （404bp） of viral DNA segment from liver and spleen tissues, the detected rate of liver＇ s specific viral nucleic acid achieved 90% , the spleen＇ s achieved 50%. Thus these cases could be proved to infect avian lymphoid leukosis from the above experiments, and the liver＇s viral detected rate was.obviously higher than spleen＇ s through the PCR detection.
出处
《山地农业生物学报》
2008年第4期320-324,共5页
Journal of Mountain Agriculture and Biology
基金
贵阳市农业局兽医科技项目资助[(2006)5001
(2008)动防-01]
贵州省农业厅畜牧局兽医科技项目(资助2008-06)
作者简介
曹冰玉（1985-），女，贵州贵阳人，南京农业大学兽医专业在读本科生，主要从事动物疫病病原生物学研究。
通讯作者：E—mail：as．bjzhou@gzu．edu．cn
