摘要
目的观察肉苁蓉对负重游泳小鼠肝脏乳酸脱氢酶同工酶、糖原及一氧化氮合酶3(NOS3)的影响,探讨其抗运动性疲劳的作用机制。方法将昆明种小鼠随机分为正常对照组、运动对照组和肉苁蓉实验组。正常对照组和运动对照组每只每日灌胃生理盐水0.2 mL,肉苁蓉实验组每只每日灌胃肉苁蓉水煎液0.2 mL(3 g/kg),共15 d;末次给药1 h后,运动对照组和肉苁蓉实验组小鼠尾部负重5%游泳90 min,10 h后连同正常对照组小鼠处死取肝脏,一部分用中性甲醛固定制备石蜡切片,一部分制成肝细胞匀浆检测乳酸脱氢酶活性;常规HE染色观察肝脏组织结构,糖原染色法显示肝脏糖原,S-P免疫组织化学染色法检测小鼠肝脏NOS3的表达。结果运动对照组与正常对照组比较,乳酸脱氢酶活性高(P<0.05),肝脏结构损伤严重,糖原含量减少,NOS3表达下降(P<0.05);肉苁蓉实验组与运动对照组比较,乳酸脱氢酶活性降低,主要是乳酸脱氢酶5(LDH5)下降,肝小叶结构较清晰,糖原丰富,NOS3表达上调(P<0.05)。结论肉苁蓉可降低LDH5活性,上调NOS3的表达,促进肝糖原合成,发挥肝脏保护和促进体能恢复作用。
Objective To study the effect of cistanche deserticola on lactate dehydrogenase （LDH） isoenzyme, glycogen and nitric oxide synthase 3 （NOS3） of liver in the mice burden swimming and explore relevant molecular mechanisms of anti-sports fatigue. Methods The mice were devided into the normal control group, the sport control group and the cistanche deserticola experimental group. Each mice of the normal control group and the sport control group was given saline 0.2 mL per day. Each mice of the cistanche deserticola experimental group was given water decoction of cistanche deserticola 0.2 mL （3 g/kg） per day. The administrations were for 15 days. The burden swimming for 90 minutes was carried out on the mice of the sport control group and the cistanche deserticola experimental group at 1 hour after the last administration. Livers of the mice were removed after 10 hours of swimming. One part of liver was fixed in the neutral formalin liquid to prepare the paraffin sections and the others was used for measurement of LDH activity. The structure of the liver was observed by staining of HE and the liver glycogen were measured by staining of glycogen. NOS3 was examined by S-P immunohistochemical method. Results The liver structure of the sport control group was injured seriously, the isoenzyme of LDH4 and LDH5 were higher, the liver glycogen were poor, NOS3 was decreased compared with the normal control group （P〈0.05）. The liver structure of the cistanche deserticola experimental group was good, LDH5 isoenzyme was low, the glycogen was rich and expression of NOS3 was upregulated compared with the sport control group （P〈0.05）. Conclusions Cistanche deserticola could decrease LDH5, protect the liver of the mice burden swimming and accelerate glycogen accumulation by increasing expression of NOS3 to protect liver and improving physical capacity recovery.
出处
《中国中医药信息杂志》
CAS
CSCD
2008年第4期24-25,28,共3页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
肉苁蓉
肝糖元
一氧化氮合酶3
小鼠
cistanche deserticola
liver glycogen
nitric oxide synthase 3
mice
作者简介
通讯作者：闰晓红，Tel：0471-6636297，E-mai1：hongxinydiy@yahoo．com
