摘要
利用细胞培养方法从长春某动物园送检的死亡川金丝猴心脏中分离获得1株柯萨奇病毒,经系统的形态学、理化学、动物回归试验和RT-PCR扩增,符合柯萨奇B型病毒特征;进而对其VP1基因部分序列和特异性血清抗体分析,证明所分离的病毒为柯萨奇B3型病毒。这是国内外首次从金丝猴分离到该病毒,暂命名为CVB/SGM-05。
A coxsackievirus B strain was successfully isolated by cells culture from cardiac muscle tissues of a dead Sichuan golden monkey with myocarditis from a zoo of Changchun in China. The isolate was consistent with CVB by morphology, physicochemistry test, animal regression test and RT-PCR. Analysis of VP1 partial gene sequence and detection of mice specific serum IgG showed that the strain isolated was a coxsackievirus B3. It was the first CVB case report in Sichuan golden monkey and the strain isolated was named CVB/SGM-05.
出处
《病毒学报》
CAS
CSCD
北大核心
2008年第4期312-316,共5页
Chinese Journal of Virology
基金
高等学校博士学科点专项科研基金(20060183070)
关键词
柯萨奇病毒
分离鉴定
川金丝猴
coxsackievirus
isolation and identification
Sichuan golden monkey
作者简介
贺文琦(1977-),男,内蒙古呼和浩特人,在读博士,讲师,主要从事动物分子病理学和病毒学研究。E-mail:hwq_jldx—b12005@yahoo.com.cn。Tel:86-431-87835376
通讯作者:高丰,教授,博导,E-mail:gf_jldxnxb@yahoo.com.cn