摘要
以诸葛菜为材料,对高温处理诱导诸葛菜花粉染色体加倍进行了研究.结果表明:诸葛菜花蕾长度与小孢子母细胞减数分裂具有相关性;当花蕾长度在0.22～0.26cm时,以小孢子母细胞减数分裂终变期分裂相所占比例最大,此时在38～45℃高温下持续处理2～4h,可诱导花粉染色体加倍的未减数2n花粉,2n花粉平均诱导率最高为29.4%,个别处理甚至高达42.5%.研究进一步证明,高温等环境剧变可以诱导植物染色体倍性变异,在植物多倍化的进化中具有积极意义.
Chromosome doubling of Orychophragrnus violaceus L. was induced by high temperature. The results indicated that there is a correlation between meiosis of microsporocyte and flower buds＇ length. In 0. 22-0.26 cm long buds,the most microsporocytes were in diakinesis,which is the best optimal stage for chromosome doubling. Buds in meiotic stages suited for heat treatment at 38-45℃ for 2 to 4 hours, the highest average proportion of 2n pollen produced rate was 29.4% and in one of the experiment treats 2n pollen even reached to 42.5 %. The research suggest that high temperature and other environment upheaval can inducing plant chromosome doubling,and this is a great significance to plant evolution via polyploidization.
出处
《西北植物学报》
CAS
CSCD
北大核心
2008年第6期1128-1133,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30671708)
关键词
诸葛菜
高温
花粉染色体加倍
进化
Orychophragrnus violaceus L.
high temperature
pollen chromosome doubling
evolution
作者简介
李悦（1983-），女（汉族），硕士研究生，主要从事林木遗传育种研究。E-mail：boopa@163．com
通讯作者：康向阳，博士，教授，博士研究生导师，主要从事林木倍性育种与细胞遗传学研究。E-mail：kangxy@bjfu．edu．cn
