摘要
目的探讨黄芪对慢性应激大鼠生长和脑单胺类神经递质的影响。方法将15只大鼠分为对照组、应激组和黄芪组。应激组和黄芪组均接受7 d的应激刺激,黄芪组再用黄芪灌胃(0.159 g/d)10 d,取3组大鼠的脑组织,用高效液相色谱法测定高香草酸(HVA)、去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)的含量。结果应激组大鼠日增重明显低于对照组,黄芪组大鼠日增重明显高于应激组。应激组大鼠海马、纹状体和前额叶中HVA、NE、DA和5-HT与对照组间差异无统计学意义。黄芪组大鼠纹状体中DA含量明显高于应激组,前额叶中DA明显低于应激组。结论黄芪对慢性应激引起的大鼠生长受抑有缓解作用,对其脑内单胺类神经递质有部分调节作用。
Objective To investigate the effects of Astragalus merrdoranaceus （ Fisch. ） Bunge. on growth and brain monoamine neurotransmitters in chronic stressed rats. Methods Fifteen individuals were divided into three groups： the control, the stressed and the Astragalus membranaceus （Fisch.）Bunge. groups. Rats in both the stressed and Astragalus membranaceus （Fisch.） Bunge. groups had stress for 7 days, and rats in the later group were fed with 0. 159 g/d Astragalus membranaceus （ Fisch. ） Bunge. for 10 days after being stressed. The contents of homovanilhc acid （HVA）, norepinephrine （NE）, dopamine （DA） and 5-hydroxytryptamine （5-HT） were determined by high performance liquid chromatogram （HPLC）. Results The daily growth mass in the stressed group was significantly decreased compared with the control group, while that of the Astragalus membranaceus （ Fisch. ） Bunge. group was higher than the stressed group. The HVA level of the hippocampus, striatum and prefrontal lobe had no statistical difference between the stressed group and the control group however they had an increase tendency in the stressed group, and the DA level of the striatum in the Astragalus membranaceus （ Fisch. ） Bunge. group was significantly higher than that of the stressed group, however, the DA level of the prefrontal lobe was significantly decreased compared with the stressed group. Conclusion Astragalus membranaceus （ Fisch. ） Bunge. alleviates the decrease of growth induced by stress, and partly regulates the monoamine neurotransmitters level of brains in stressed rats.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第7期645-649,共5页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金资助项目(Z-2002DO4)
关键词
大鼠
应激
黄芪属植物
神经递质
Rats
Stress
Astragalus plant
Neurotransmitters
作者简介
张峰（1964-），女，理学硕士，副教授，主要从事植物化学研究。
通讯作者：李法曾（1941-），教授，博士生导师。E-mail：lifz@sdnu．edu．cn
