摘要
背景:国内外研究表明镍钛合金在体内长期置入可释放镍离子,引起血浆镍含量增高。目的:通过大体、扫描电镜及血浆镍含量的测定,了解左心耳封堵器置入后封堵材料表面的内皮化效应。设计、时间及地点:对比观察实验,于2005-09/2006-08在南京医科大学第一附属医院江苏省实验动物中心完成。材料:选用南京农科院种猪养殖厂提供的苏钟小型种猪17只,随机分为实验组12只和对照组5只。方法:实验组使用自主研制的镍钛合金左心耳封堵器行左心耳封堵,对照组手术步骤相同而不施行左心耳封堵。主要观察指标:封堵前、封堵后1,3,6个月取外周静脉血浆,采用石墨炉原子吸收分光光度法测定镍离子含量;大体及扫描电镜观察封堵器表面内皮细胞生长情况。结果:实验组血浆镍含量于封堵后1个月达高峰,与术前相比差异有显著性意义(P<0.001),封堵后6个月基本回复至术前水平,与术前相比差异无显著性意义(P>0.05);大体及电镜结果表明封堵器表面内皮化在封堵后4周基本完成。结论:镍离子可以从封堵器表面释放导致血循环引起血浆镍含量升高,而封堵材料表面内皮化的形成阻碍镍离子的进一步释放。
BACKGROUND： Many studies have demonstrated that nickel-titanium alloy can release nickel ion after long-term implantation in vivo, and induce an increase in plasma nickel content. OBJECTIVE： To detect the endothelialization on the surface of a novel nickel-titanium shape memory alloy-made left atrial appendage occluder through gross and scanning electron microscopic observations and plasma nickel content measurement. DESIGN, TIME AND SETTING： Comparative observation was performed at the Experimental Animal Center of First Hospital of Nanjing Medical University from September 2005 to August 2006. MATERIALS： Seventeen Suzhong miniature pigs （Nanjing Academy of Science and Agriculture） were randomly divided into experimental group （n=12） and control group （n=5）. METHODS： The nickel-titanium shape memory alloy-made left atrial appendage occluder was implanted into the experimental pig surgically. While the control pigs underwent the same operation procedure and the occluder was not implanted. MAIN OUTCOME MEASURES： Atomic absorption spectrometry was used to measure nickel ion concentration in peripheral vein plasma before, 1, 3, and 6 months after occlusion. Gross examination and scanning electron microscopic were used to evaluate the adherence and growth of endothelial cells to the surface of the occluder. RESULTS： Plasma nickel ion concentration reached the peak one month after surgery in the experimental group, significantly different from that before surgery （P 〈 0.01）, and restored to preoperative level 6 months after occlusion （P 〉 0.05）. Gross and scanning electron microscopic observations showed that endothelialization on the occluder surface completed at 4 weeks after occlusion. CONCLUSION： Nickel ion seems to be released from the occluder, causing a systemic rise in serum levels of nickel. Endothelialization of the device surface inhibits nickel release.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第26期5042-5046,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
作者简介
史爱武，女，1970年生，江苏省南京市人,汉族，2007年南京医科大学毕业，博士，主治医师，主要从事心血管内科的临床工作。shiaiwu888@163.com
