摘要
目的:观察联合应用热休克蛋白70(HSP70)与卡介苗(BCG)对Hcaf肿瘤的抑制作用,为应用肿瘤来源的HSP70治疗人类恶性肿瘤提供参考依据.方法:实验小鼠64只随机分为对照组、BCG免疫组、HSP70免疫组和联合组4组,每组16只.小鼠接种Hcaf细胞制成肿瘤模型.BCG组接种1mgBCG;HSP70组注射10μg HSP70,联合组以HSP70 10μg和BCG1mg代替,对照组注射50μL PBS液.观察小鼠的生存时间、生存率和肿瘤大小等指标的变化.取肿瘤组织进行免疫组化实验,观察肿瘤细胞性状及AFP的表达情况.结果:应用免疫治疗后,小鼠肿瘤生长均得到不同程度的的抑制,生存期均有延长,其中HSP70组和联合组与对照组相比差异显著(77.1±15.1d,82.8±13.2dvs22.9±3.50d,均P<0.01),同时生存率也有所提高(41.7%,66.7%vs0,bothP<0.01).对照组及BCG免疫组AFP表达明显,而HSP70组和联合组AFP表达均受到不同程度抑制,其中联合组尤其显著.与对照组及BCG免疫组比较,HSP70组和联合组肿瘤细胞异型性均有所降低.结论:HSP70与BCG联合应用对Hcaf细胞有明显抑制作用,其疗效要优于单独应用HSP70或BCG.
Abstract AIM： To investigate the anti-Hcaf cell efficacy induced by heat shock protein 70 （HSP70） and bacillus calmette-guerin （BCG）, and to provide references for treatment of human malignant tumor using HSP70.
METHODS： Sixty-four mice were randomly and averagely divided into 4 groups： control group （injected with 50 μL PBS）, BCG group （immunized with Img BCG）, HSP70 group （immunized with 10 μg HSP70） and combination group （immunized with 10 μg HSP70 plus 1 mg BCG）. Rat tumor model was induced by inoculation of Hcaf cells. Survival time, survival rate and tumor size were observed evaluated. Tumor tissues collected to observe the characteristics of Hcaf cells and alpha-fetoprotein （AFP） expression.
RESULTS： After immune treatment, the tumor growth of mice was inhibited, and the survival time was significantly prolonged in the HSP70 group and the combination group as compared with that in the control group （77.1 ± 15.1 d, 82.8 ± 13.2 d vs 22.9 ± 3.50 d, both P 〈 0.01）; meanwhile, the survival rate was also enhanced （41.7%, 66.7% vs 0, both P 〈 0.01）. AFP was expressed markedly in the control group and the BCG group; however, it was restrained in the HSP70 group and the combination group, especially in the latter. In comparison with that in the control group or the BCG group, the tumor cell atypia was decreased in the HSP70 group and the combination group.
CONCLUSION： Combination of HSP70 and bacillus calmette-guerin can inhibit the growth of Hcaf cells remarkably, which is superior to HSP70 or BCG alone.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第17期1855-1858,共4页
World Chinese Journal of Digestology
关键词
热休克蛋白70
卡介苗
Hcaf肿瘤细胞
免疫组化
Heat shock protein 70
Bacillus calmette-guerin
Hcaf tumor cell
Immunohistochemis-try
作者简介
杨野，医学博士，主要从事肝胆肿瘤的外科治疗。通讯作者：杨野，110001，辽宁省沈阳市，中国医科大学附属第一医院干诊科．doctor_3047_cn@sina．com．cn 电话：024—23256666
