摘要
对不同来源的何首乌在组织培养中的表现进行了比较研究。结果表明,不同来源的何首乌在组织培养中的表现有较大的差异,其中采自广西农科院的茎段在不定芽的诱导、不定芽的高度、叶面积大小等方面均是最优的。在愈伤组织的诱导与生长方面,当以茎段为诱导材料时,以来源于广西农科院的材料最好;当以叶片为诱导材料时,以来源于广西凌云的材料最好。
The tissue culture of Polygonum multiflorum Thunb,from different regions was studied in this paper.The results showed that there was a great difference in the performance of tissue culture between different sources,The stem section collected from the Guangxi Institute of Agricultural Sciences was greater than the others in adventitious bud's induction,height and leaves area.In inducement and growth of callus,the best one was the stem collected from Guangxi Institute of Agricultural Sciences if the stems were used as inducement materials;while the leaves collected from Lingyun in Guangxi province were the best if using leaves as the materials.
出处
《林业科技开发》
2008年第4期91-93,共3页
China Forestry Science and Technology
基金
广西教育厅资助项目“利用组织培养技术培育何首乌多倍体”(编号:200510003)
关键词
何首乌
组织培养
不同来源
Polygonum multiflorum Thunb
Tissue Culture
Different Regions
作者简介
第一作者:韦娜(1983-),女,硕士生,研究方向为药用植物栽培与繁殖;通讯作者:杨振德,男,教授。E-mail:dzyang68@126.com