摘要
为了研究大蚕蛾科绢丝昆虫的进化关系,分别从蓖麻蚕、樗蚕和柳蚕的蛹中提取线粒体DNA,用PCR方法扩增线粒体A+T丰富区及侧翼序列,克隆到pMD18-T载体,进行序列测定.序列分析表明,克隆片段的基因依次为12SrRNA基因3′端、A+T丰富区、tRNAMet、tRNAIle、tRNAGln和ND2基因5′端,与家蚕和野桑蚕mtDNA排列顺序相同;在tR-NAGln和ND2基因之间有一段非编码区.根据柳蚕3个tRNA基因的核苷酸序列推定了其二级结构.基于5种大蚕蛾科绢丝昆虫和家蚕mtDNA A+T丰富区序列的系统发育树显示,柳蚕与蓖麻蚕、樗蚕的亲缘关系较近,与柞蚕的亲缘关系较远,而与家蚕的亲缘关系更远.本研究结果有助于阐明大蚕蛾科绢丝昆虫以及家蚕的起源和进化.
To study the phylogeny of silkmoths of Saturniidae, the mitochondrial DNAs （mtDNAs） were extracted from the pupa of Philosamia cynthia ricini ,Philosamia cynthia and Actias selene ningdoana respectively. The A ＋T-rich regions and flanking sequences were amplified with the polymerase chain reaction （PCR） method, and cloned into the pMD18 - T vector and then sequenced. Results from sequence analysis show that gene orders in all cloned fragments are 3＇ -end portion of 12S rRNA, A ＋ T- rich region, tRNA^Met, tRNA^Ile, tRNA^Gla and 5＇ - end portion of ND2, which are the same as those of Bombyx mori and Bombyx mandarina. Between tRNAGIn and ND2 there is a non - coding region. The secondary structures of three tRNAs of A. s. ningdoana are deduced based on the nucleotide sequences. The phylogenetic tree based on the A ＋ T-rich regions from 5 species of Saturniidae and 2 varieties of B. mori reveals that A. s. ningdoana is closer to P. c. ricini and P. c. cynthia, a little far from Antheraea pernyi, but remote to B. mori in blood relationship. These results will benefit to the study of the origins and the molecular evolution of Saturniidae insects as well as B. mori.
出处
《江苏科技大学学报（自然科学版）》
CAS
北大核心
2008年第3期66-71,共6页
Journal of Jiangsu University of Science and Technology:Natural Science Edition
基金
国家重点基础研究发展计划"973"资助项目(2005CB121000)
关键词
大蚕蛾科
非家蚕
线粒体DNA
A+T丰富区
系统发育树
saturniidae
non-mulberry silkworms
mitochondrial DNA
A ＋ T-rich region
phylogenetic tree
作者简介
沈兴家（1963-），男，浙江嵊州人，研究员，博士生导师，研究方向为家蚕生物化学与分子生物学．E-mini：shenxj@sficaas．com.
