摘要
目的:观察神经细胞黏附分子(NCAM)在大鼠不同发育时期视皮质中的表达及其在视觉发育中的作用。方法:42只Wistar大鼠,按出生后天数分成3、14、21、30、45、60、90d共7组,取视皮质,应用免疫组织化学方法和计算机图像分析技术,测定大鼠视皮质NCAM蛋白的表达。结果:NCAM在各组大鼠视皮质均有表达。大鼠越年幼,表达水平越高,随着日龄增加,表达水平相对下降,到发育晚期或成熟期其表达明显下降。结论:大鼠视皮质NCAM表达随发育呈下降的趋势,属于发育相关性蛋白。
Objective： To investigate the expression and role of NCAM in visual cortexes of the rats during different developmental stages. Methods： Forty-two Wistar rats were randomly divided into seven groups including 3 d, 14 d, 21 d, 30 d, 45 d, 60 d and 90 d groups. All the visual cortexes were taken from them, and the expression of NCAM was determined by immunohistochemistry. The immunohistochemistry stainings were analyzed by automatic image analysis technology. Results： The expression of NCAM in the visual cortexes were present in all ages. The expression was obviously dropped with the age increase. Conclusion： NCAM is a developmental related protein in visual cortex.
出处
《天津医药》
CAS
北大核心
2008年第6期441-443,I0006,共4页
Tianjin Medical Journal
基金
天津市科委自然科学基金资助项目(项目编号:05YFJMJC12400)
作者简介
通讯作者E-mail：ZKX@tijmu．edu．cn
