摘要
以40年生砀山酥梨为材料,用ZD-IA型照度计测定光照强度,用分光光度法测定POD、PAL、PPO等3种酶的活性;研究了光照强度对果实发育过程中POD、PAL、PPO等3种酶活性的影响。结果表明:(1)光照强度对POD、PAL、PPO等3种酶活性有显著的影响;(2)在光照充足的部位,POD、PAL酶活性较高,具体表现为高光强>中光强>弱光强>极弱光强,彼此间活性差异极显著(P<0.01);(3)PPO在光照充分的情况下,活性偏低,表现为高光强<中光强<弱光强<极弱光强,差异显著。
The test samples are collected from 40 years old Pyrus bretschneideri cv. Dangshan Su. The light intensity is measured with the ZD-ZA illumination meter, and the activity of POD, PAL and PPO is tested by colorimetry with spectrophotometer. This test studies the influence of light intensity on POD, PAL and PPO activity of Pyrus bretschneideri cv. Dangshan Su fruit in its growth phase. The experiment shows ： （ 1 ） Light intensity has noticeable influence on POD, PAL and PPO activity. （2） With enough light intensity, POD, PAL shows high activity, the activity value with high light intensity 〉 middle light intensity 〉 thin light intensity 〉 the thinnest light intensity. Meanwhile, the divergence of activity between each other is remarkable（ P 〈 0.01 ）. （ 3 ） With enough light intensity, PPO shows low activity, with the trend of high light intensity 〈 middle light intensity 〈 thin light intensity 〈 the thinnest light intensity. Besides, the difference is also remarkable.
出处
《激光生物学报》
CAS
CSCD
2008年第3期295-298,共4页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(30771483)
安徽省教育厅自然科学研究项目(2002kj297)
宿州学院教授(博士)基金项目(2006jb06)
关键词
光强
砀山酥梨
POD
PAL
PPO
light intensity
Pyrus bretschneideri cv. Dangshan Su
POD
PAL
PPO
作者简介
刘小阳（1963-），男，安徽怀宁人，副教授，主要从事植物生理学方面的教学和研究工作。（电子邮箱）szliuxy@sohu．com
