摘要
通过对金鱼(Carassius auratus var.)的5个代表品种草金(grass goldfish)、红龙睛(red dragoneye goldfish)、鹤顶红(red-head wen goldfish)、水泡(blisters-eyegoldfish)、黑寿(white-head oval goldfish)线粒体DNA细胞色素b基因全序列进行测定,结果发现金鱼Cytb基因全序列1141bp,其中T、C、A、G4种碱基含量分别为29.1%,27.8%,28.4%,14.5%,A+T的含量(57.8%)高于C+G的含量(42.2%)。草金、红龙睛、鹤顶红、水泡、黑寿Cytb基因全序列一样。结果表明,细胞色素b基因在金鱼种内是相当保守的,草金、红龙睛、鹤顶红、水泡、黑寿起源于同一祖先。将鲫鱼和金鱼线粒体Cytb基因序列比较分析,同源性达97.9%,应该还在同一种的水平上。
Mitochondrial cytochrome b DNAs of representative varieties of Carassius auratus var. , viz. grass goldfish, red dragoneye goldfish, red-head wen goldfish, blisters-eye goldfish and white-head oval goldfish were sequenced. 1 141 bp of mitochondril cytochrome b gene sequence has been obtained from C. auratus var. and the content of T, C, A, G of the gene were 29.1%, 27.8%, 28.4% , 14. 5% , respectively. The sequences were same in those varieties of C. auratus var. So the results showed that Cyt b gene was conservative and indicated grass goldfish, red dragoneye goldfish, red-head wen goldfish, blisters-eye goldfish, white-head oval goldfish may be the same species. According to the analysis, C. auratus var. is relatively closer to C. auratus. Sequence analysis of mtDNA Cyt b gene of C. auratus var. and C. auratus which has 97. 9% homology suggests it may be reasonable in a species.
出处
《南方水产》
2007年第1期26-30,共5页
South China Fisheries Science
基金
广东省农业攻关计划项目(2005B20301015)
关键词
金鱼
鲫鱼
细胞色素B基因
亲缘关系
Carassius auratus var.
C. auratus
Cyt b
phylogenetic relationship
作者简介
牟希东（1981-），男，硕士研究生，从事遗传育研究种。E-mail：muxd1019@163．com
通讯作者：罗建仁，E-mail：fishlo@163．net
