摘要
目的观察戊四氮点燃致痫发育期大鼠模型行为改变和脑内突触重建情况及天麻素对其影响。方法将50只21日龄Wistar发育期大鼠（50-70g）随机分为5组：对照组、戊四氮组、天麻素大剂量组、天麻素小剂量组及丙戊酸钠组,每组10只。根据Racine分级法,观察大鼠行为学改变,记录大鼠痫性发作级别和次数。在点燃4周后常规心脏灌注、固定及取脑组织,采用免疫组织化学方法检测各组大鼠组织切片突触体素（P38）的表达。结果1.戊四氮点燃4周后,各实验组大鼠痫性发作级别比较具有统计学意义（χ^2=35.83P〈0.01）,戊四氮组发作级别最高（平均秩为44.60）,其次是天麻素小剂量组（平均秩为34.90）、天麻素大剂量组（平均秩为21.45）、丙戊酸钠组（平均秩为19.55）。2.戊四氮点燃4周后,各组实验大鼠颞叶皮质及海马区均有P38表达,戊四氮组P38表达量较其他组多,有统计学差异（Pa〈0.01）,天麻素大剂量组表达量小于天麻素小剂量组（q=2.705,2.351Pa〈0.05）,天麻素大剂量组P38表达量与丙戊酸钠组比较均无统计学意义（q=1.194,1.031Pa〉0.05）。结论戊四氮反复点燃可导致发育期大鼠颞叶和海马区苔藓纤维发芽和突触重建的形成;天麻素具有明显抗癫痫作用,可通过降低P38的表达,抑制突触重建的形成,间接控制癫痫发作。
Objective To observe the behavior changes and synaptic reconstruction of brain and the impact of gastrodin in rats with epilepysy induced by pentylenetetrazole. Methods Fifty Wistar rats in growth（50 -70 g） were randomly divided into 5 groups：control group, Pentylenetetrazole （PTZ） group, gastrodin high dose group, gastrodin low dose group and sodium valproate group, each group had 10 rats. According to Racine classification,the behavior changes of rats and the frequency were recorded. Conventional method was adopt to perfuse cordis, fix and extract the brain, and the immunohistochemistry was used to detect synaptophysin（ P38 ） expression in the temporal lobe and hippocampus of the brain after 4 weeks. Results 1. Four weeks after pentylenetetrazole ignited the growth period rats,the attack - level of each experimental group through comparison with each other, there was statistically significant difference （ Χ^2 = 35.83 P 〈 0.01 ）, the attack - level of PTZ group was highest of all（ Mean Rank = 44.60 ）, followed by low dose gastrodin group （ Mean Rank = 34.90 ）, high dose gastrodin group （ Mean Rank = 21.45 ）, sodium valproate group （ Mean Rank = 19.55 ）. 2. Four weeks after pentylenetetrazole ignited the growth period rats, P38 expression of PTZ group was more than other groups in temporal lobe cortex and hippocampus （Pa 〈 0.01 ）, and P38 expression in high dose gastrodin group was more than that in low dose gastrodin group （ q = 2. 705,2.351 Pa〈 0.05 ） , however, for P38 expression in high dose gastrodin group with sodium valproate group,there were no statistical differences （ q = 1. 194,1.031 Pa 〉 0.05 ）. Conclusions There are mossy fiber sprouting and the formation of synaptic reconstruction in temporal lobe and hippocampus of the growth period rats repea- tedly ignited by pentylenetetrazole. Gastrodin may play a role in the formation of antiepileptic obviously,and through decreasing the expression of P38,which can inhibit the formation of synaptic reconstruction,as control seizures indirectly.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2008年第10期776-778,共3页
Journal of Applied Clinical Pediatrics
基金
甘肃省科技厅自然科学基金项目资助(3ZS051-A25-087)
关键词
癫痫
戊四氮
突触体素
突触重建
天麻素
epilepsy
pentylenetetrazole
synaptophysin
synaptic reconstruction
gastrodin
作者简介
曹亚芹，女，硕士生，研究方向为小儿癫痫。
通信作者：苏怡凡，女，主任医师，硕士生导师，研究方向为神经系统疾病，电子信箱yifansu0931@126．com
