摘要
目的探讨基质金属蛋白酶MMP-2和MMP-9与多发性肌炎，皮肌炎（PM／DM）的关系。方法采用实时荧光定量PCR和Western印迹方法分别检测PM／DM患者外周血单一核细胞（PMBC）中MMP-2和MMP-9的mRNA和蛋白表达水平。结果MMP-2、MMP-9在PM／DM患者外周血PMBC中的mRNA表达水平明显高于正常人对照组，其中MMP-2为正常人对照组的2．41倍、MMP-9则为1．66倍。Western印迹检测发现MMP-2、MMP-9在PM，DM患者外周血PMBC中的蛋白表达水平也明显高于正常人对照组，与其mRNA表达结果一致。结论基质金属蛋白酶MMP-2和MMP-9可能在PM／DM的发生中起作用。
Objective To investigate the relation of the expressions of matrix metalloproteinase-2 （MMP-2） and -9 （MMP-9） with polymyositis/dermatomyositis （PM/DM）. Methods Blood samples were obtained from 8 patients with PM, 12 patients with DM and l0 normal human controls. Real-time quantitative RT-PCR and Western-blot were applied to examine the mRNA and protein expressions of MMP-2 and MMP-9 respectively in the peripheral blood mononuclear cells （PBMC） of these patients. Results The mRNA expression levels of MMP-2 and MMP-9 in the PBMC of patients with PM or DM were 2.41 and 1.66 folds as high as those of the normal controls, respectively; these differences were significant between the two groups. Increased expressions of MMP-2 and MMP-9 were also noted in the patients with PM or DM. Conclusion MMP-2 and MMP-9 may play a part in the development of polymyositis and dermatomyositis.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2008年第6期397-399,共3页
Chinese Journal of Dermatology
基金
湖南省科学技术厅科技计划基金（06FJ3150）
