摘要
大肠杆菌主要寄居于人和动物的肠道内,在自然界分布广泛,属于条件致病菌。大肠杆菌能通过消化道使人类和畜禽发生感染和中毒。对大肠杆菌病的防治和流行病学调查都依赖于对病原的及时准确的检测和鉴定。本文对大肠杆菌病各种实验室病原检测方法,包括细菌的分离培养、荧光免疫测定技术、免疫胶体金标记技术、免疫磁珠分离法、多重PCR、荧光定量PCR等方法作以概要性综述。
Escherichia coli widely spread in the nature especially parasitized in intestinal tract of human and animals, which belong to conditioned pathogen. It can infect human, livestock and poultry by digestive tract, sometime can induce disease. In this review, we try to recommend some diagnostic methods of Escherichia coli used in laboratory. They are bacteria isolation and culture, Fluorescent Immunoassay, Immunologic Colloidal Gold Signature, Immunomagnetic Bead Separation, Multiple PCR, Fluorescence Quantization PCR and so on. This review provided the reference for the diagnosis and therapy of Escherichia coli by comparing between advantage and disadvantage of above-mentioned diagnostic methods.
出处
《黑龙江八一农垦大学学报》
2008年第2期51-54,共4页
journal of heilongjiang bayi agricultural university
作者简介
冉旭华（1978-），女，讲师，中国农业科学院博士研究生毕业，现主要从事动物病毒分子免疫学的教学与科研工作。
通讯作者：闻晓波，男，讲师，E-mail：wenxiaobo1977@163.com。
