摘要
目的调查深圳地区不孕妇女的生殖道沙眼衣原体(CT)和解脲支原体(UU)感染;比较荧光定量PCR法与UU培养或金标法检测CT的敏感性。方法219例不孕妇女患者取宫颈分泌物,用荧光定量PCR法检测219例分泌物标本UU的DNA,同时对196例标本进行CT的DNA检测。另选68例正常体检女性作为对照组;同时采用支原体培养药敏试剂盒进行UU培养以及金标法检测CT,并对结果进行统计、分析。结果荧光定量PCR检出UU阳性率39.7%(87/219),CT阳性率44.9%(88/196),分别高于对照组(P<0.05)。同时,培养法检出UU阳性率为29.7%,金标法检测CT阳性率为27%,与PCR相比,P<0.05。结论输卵管不孕妇女生殖道UU和CT检出率较高,荧光定量PCR具有敏感性高和特异性强的特点,对不孕患者CT和UU的诊疗具有实际意义。
Objective To survey the infectious status of Ureaplasma urealyticum （UU） and Chlaydia trachomatis （CT） in genitourinary tract of women in Shenzhen and explore the 1 value of FQ - PCR in detection of UU and CT. Methods Real time fluorescent quantitative polymerase chain reaction（ FQ - PCR） was used to detect UU and CT, and UU was cultured and detected. Results The positive rate of CT （ 44. 9% ） or UU（39.7%） by FQ - PCR was higher than that of the control （P 〈 0.05 ）. The positive rate of UU by FQ - PCR was 39.7%, higher than that of cultivation （29.7%）. The positive rate of CT was 44.9% by FQ - PCR, higher than that of gold - labelled antigen detection method （ 27% ）. Conclusion UU and CT can be detected in endocervices of infertile women. It is suggested that FQ - PCR shows practical value in diagnosis and treatment of mycoplasma infection or Chlamydia trachomatis infection.
出处
《中国热带医学》
CAS
2008年第6期1030-1031,共2页
China Tropical Medicine
关键词
不孕
解脲支原体
沙眼衣原体
金标法
荧光定量PCR
Infertility
Chlaydia trachomatis
Gold - labelled antigen detection method
FQ - PCR
Infertility
