摘要
以云南红豆杉为实验材料,比较了石蜡切片法、洋红简易染色法和整体染色透明法研究小孢子发生过程的效果,分析了用石蜡切片法及离体培养法研究雄配子体发育优缺点.在离体培养中,对比了不同的蔗糖质量浓度、培养温度及染色方法对实验结果的影响,从而为针叶树种雄性生殖系统发育过程研究提供参考.结果显示:整体透明染色法操作简单,能够反应小孢子发育过程中各个阶段的细胞结构,是用于小孢子发育过程研究的比较理想的方法;对于雄配子体发育过程的研究,需要用石蜡切片法与离体培养法相结合,在离体培养中,采用雄球花表面灭菌,自然散粉,低糖低温培养有利于防止污染,洋红染色法方便快捷,不需要特别的设备,是离体培养时比较理想的染色方法.
By using Taxus yunnanensis as experiment material, different methods including.paraffin embed, squashed with aceto-camine and stain-clearing, methods of sporogenesis, paraffin embedded samples and cultured in vitro methods for microgametogenesis were used and evaluated. In cultured in vitro methods, the influence of culture temperature, the sucrose concentration of medium and stailling methods on the results was studied. The results can be summarized as follows： stain-clearing technique is a better method for sporogenesis due to its simple operation and better results. Raffin embedded samples and cultured in vitro are necessary methods in gametogenesis,and in vitro, sterilizing on surface and releasing microspores by itself,low temperature and low sucrose concentration are helpful to eliminate contamination. It is convenient that male gametogeuesis in vitro be stained by aceto-carmine.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期302-307,共6页
Journal of Yunnan University(Natural Sciences Edition)
基金
中国科技部科研院所社会公益研究专项资助(2004DIB3J104)
中国科技部"十五"科技支撑项目资助(2006BAD18B03)
加拿大阿尔伯塔大学益客基金会资助
中国林科院资源昆虫研究所所长基金资助(riri200703Z)
作者简介
王兵益（1976-），男，陕西人，博士生，助理研究员，主要从事植物生殖生物学方面的研究．
通讯作者：苏建荣（1970-），男，云南人，博士，副研究员，主要从事植物种群生物学方面的研究．
