摘要
从一头标号为９２０４４的进口奶牛分离外周血淋巴细胞，将此淋巴细胞与正常胎牛肺细胞（ＦＢＬ）进行共培养，一个月后共培养物出现合胞体。此时电镜观察可见病毒的出芽过程。免疫染色显示，此培养物可与牛免疫缺陷病毒（ＢＩＶ）外膜蛋白的单克隆抗体特异性结合。对共培养物裂解产物进行ＰＣＲ，可分别得到ＢＩＶ反转录酶（ＲＴ）编码区和外膜蛋白编码区（ｅｎｖ）的特异性带。序列分析显示，ＰＣＲ的ＲＴ产物与美国的ＢＩＶＲ２９毒株有两个碱基的改变，从而证明我们在中国分离到一株ＢＩＶ。
A BIV isolate 92044 was isolated from a seropositive cattle in Tongxian, Beijing. This isolate was confirmed as BIV by syncytium formation assay, immunostaining assay, electron microscopic observation and PCR assay. RT and env fragments of 92044 were obtained from PCR and cloned into pUC18 separately. Two base pairs had changed in 243bp RT fragment of 92044 bysequence comparison to R29.
出处
《病毒学报》
CAS
CSCD
北大核心
1997年第4期357-364,共8页
Chinese Journal of Virology
基金
国家自然科学基金
国家教委博士点基金
关键词
牛病毒
牛免疫缺陷病毒
毒株
分离
鉴定
Bovine immunodeficiency virus (BIV), Syncytia formation, Polymerase chain reaction (PCR), Electron microscope
