摘要
目的:建立以高效液相色谱法测定卡马西平固体脂质纳米粒含量并计算包封率的方法。方法:色谱柱为KromasilC18,流动相为甲醇-水(6∶4,含0.1%三乙胺),流速为1.0mL·min-1,检测波长为285nm,进样量为20μL。结果:卡马西平检测浓度的线性范围为0.525～21.0μg·mL-1(r=0.9998);平均回收率为100.1%(RSD=1.42%);平均包封率为73.1%。结论:本方法简便、快速、重现性好,可用于该制剂的质量控制。
OBJECTIVE： To establish an HPLC method for the determination of the content and calculated its encapsulation efficiency of carbamazepine solid lipid nanoparticle （SLN） . METHODS： The separation was performed on Kromasil C18 column. The mobile phase consisted of methanol- water （6 ： 4, containing 0.1% triethylamine） with a flow rate of 1.0 mL · min ^-1. The detection wavelength was set at 285 nm and the injection volume was 20μL. RESULTS： The calibration curve was liner in the range of 0.525--21.0μg · mL^-（r =0.999 8）. The average recovery was 100.1% （RSD = 1.42%） and the average encapsulation efficiency was 73.1%. CONCLUSION： The method is simple, rapid, and reproducible, and can be used for the quality control of carbamazepine solid lipid nanoparticles.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第13期1009-1011,共3页
China Pharmacy
基金
陕西省科技攻关项目(2004K10-G4(3))
作者简介
副主任药师，硕士。研究方向：医院制剂及新制剂。电话：029—87679336。E—mail：xzxxzx13@126．com
