摘要
为获得油菜抗菌核病的抗源材料，进行了油菜抗菌核病突变体筛选方法的研究．用油菜种子未成熟胚或成熟胚在ＭＳ－１（ＭＳ＋２，４－Ｄ２ｍｇ／Ｌ＋ＩＡＡ０．２ｍｇ／Ｌ＋ＫＴ０．１ｍｇ／Ｌ）培养基上诱导油菜愈伤组织，继代４～５次后用含草酸６００ｍｇ／Ｌ的无Ｃａ２＋ＭＳ－１培养基处理２～４ｍｍ大小的愈伤组织９６ｈ，筛选获得的耐毒素愈伤组织移至ＭＳ－２（ＭＳ＋ＢＡ１ｍｇ／Ｌ＋ＩＡＡ０．２ｍｇ／Ｌ）培养基上获得再生小植株．
The procedure of screening for rapeseed mutants with resistance to stem rot (Sclerotinia sclerotiorum (Lib) De Bary) was proposed.Rapeseed calli were induced from immature or mature embryos on the solid MS medium supplied with 2,4D 2 mg/L,IAA 0.2 mg/L and KT 0.1 mg/L. After subcultured for 4 or 5 generations on the same medium as above the calli were cut into segments in size about 2～4 mm and treated with Ca2+free oxalic acid at the concentration of 600 mg/L for 96 h. The selected resistant calli were transplanted onto the solid MS media supplied with BA 1 mg/L and IAA 0.2 mg/L to regenerate plantlets. Some resistant rapeseed variants were obtained.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
1997年第6期515-519,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科委资助
关键词
油菜
菌核病
抗性
无性系筛选
rape stem rot resistance clonal screening
