摘要
目的:应用高通量组织芯片技术分析卵巢癌和正常卵巢组织中磷脂酰肌醇3激酶调节亚基p85α(phosphoinositide 3-kinase regulatory subunit p85 alpha,PI3Kp85α)、AKT2和Ki-67蛋白的表达,探讨其相关性及临床意义。方法:利用组织芯片技术结合免疫组化法检测177例卵巢癌和10例正常卵巢组织中PI3Kp85α,AKT2及Ki-67的表达。结果:PI3Kp85α,AKT2及Ki-67的总阳性表达率在卵巢癌组织中分别为70.6%,76.3%和74.5%,正常卵巢组织中分别为30.0%,10.0%和10.0%,蛋白阳性表达率的差异有统计学意义(P<0.05);在高分化卵巢癌组织中分别为43.5%,47.8%和47.8%,中分化卵巢癌组织中分别为69.4%,75.8%和74.2%,低分化卵巢癌组织中分别为78.3%,83.7%和84.8%,蛋白阳性表达率有统计学差异(P<0.01),其阳性表达率及强度随癌症恶性程度增高而增高;PI3Kp85α,AKT2与Ki-67蛋白的表达均存在相关性(P<0.01)。结论:联合分析PI3Kp85α,AKT2和Ki-67的表达对预测卵巢癌的发生和发展有重要意义。
Objective： To investigate the expression of PI3Kp85α,AKT2 and Ki-67 in normal ovarian tissues and ovarian cancer tissues by tissue mieroarray, and to determine correlations between the expression of the three proteins and clinical significance for ovarian cancer progression. Methods： A tissue mieroarray containing 177 specimens from ovarian cancer tissues and 10 specimens from normal ovaries were constructed, and detection of the three proteins （PI3Kp85α AKT2 and Ki-67） was accomplished by immunohistoehemistry of consecutive formalin-fixed tissue mieroarray sections. Results： The percentage of positive staining for PI3Kp85α, AKT2 and Ki-67 was 70.6%, 76.3% and 74.5%, respectively, in ovarian cancers, compared to 30.00%, 10.00% and 10.00% in normal ovarian tissues. Furthermore, 43.5%, 47.8% and 47.8% of the tissue was positive in well-differentiated ovarian cancers, 69.4%, 75.8% and 74.2% in moderately-differentiated ovarian cancers, and 78.3%, 83.7% and 84.8% in poorly-differentiated ovarian cancers. There were significant differences in expression between normal ovarian tissues and ovarian cancer tissues （P〈0.05）, with the positive expression rate and intensity being higher in the latter. There were also significant differences among well-differentiated, moderately-differentiated and poorly-differentiated ovarian cancer tissues （P〈0.01）,with the more differentiated cancers having lower expression and intensity levels. A significant correlation was detected among the expression of PI3Kp85α, AKT2 and Ki-67. Conclusion： Combination analysis of PI3Kp85α AKT2 and Ki-67 may be a practical new method for predicting the prognosis of ovarian cancers.
出处
《国际妇产科学杂志》
CAS
2008年第2期148-150,F0003,共4页
Journal of International Obstetrics and Gynecology
