摘要
目的研究薯蓣皂苷对培养乳鼠心肌细胞缺氧/复氧损伤的保护作用并探讨其作用机制。方法采用体外培养乳鼠心肌细胞,建立缺氧/复氧损伤模型,以薯蓣皂苷进行干预。心肌细胞随机分为5组:空白对照组(C组);缺氧/复氧组(A/R组);薯蓣皂苷(Dioscin)低、中、高剂量干预组(Dioscin+A/R组)。分别观察各组心肌细胞搏动频率;MTT法测细胞存活率;用Rh123荧光探针标记线粒体,检测荧光强度以反映线粒体膜电位(△Ψm)变化;Fluo-3负载心肌细胞,激光扫描共聚焦显微镜观察细胞内钙离子浓度变化。结果薯蓣皂苷干预组心肌细胞搏动频率、细胞存活率、△Ψm与A/R组比较明显升高;细胞内平均钙离子荧光强度显著低于A/R组。结论从细胞水平初步证实薯蓣皂苷预处理心肌细胞对A/R损伤有一定保护作用,保护机制可能涉及对线粒体膜保护和防止细胞内钙超载等。
Objective To investigate the mechanisms of A/R and the effects of dioscin on the cul- tured myocardial cells. Methods Cultured myocardial cells of neonatal Wistar rats were randomly divided into five groups： Control group; A/R group; dioscin L, M, H group. At the end of experiment, myocardial cells beating rate, cell viability, alteration of mitochondria membrane potential （△ψm） on myocardial cells were determined by Rh123 staining method, alteration of [Ca^2＋ ] i on myocardial cells were determined by LSCM. Result Compared with A/R group , the beating rate, cell viability and △ψm of each dioscin group was significantly increased. Compared with AZR group, the average fluorescence intensity of [ Ca^2＋ ] i of each dioscin group was significantly reduced. Conclusion It is suggested that dioscin may be involved in the process of protection on cultured myocardial cells in A/R injury. The protection of mitochondria membrane and prevention of calcium overload may be involved in the mechanism of dioscin-protection.
出处
《中国分子心脏病学杂志》
CAS
2008年第2期72-74,共3页
Molecular Cardiology of China
关键词
薯蓣皂苷
缺氧/复氧损伤
心肌细胞
线粒体膜电位
钙超载
Dioscin
Anoxia/reoxygenation （A/R） damage
Cardiomyocytes
Mitochondria mem- brane potential
Calcium overload
