摘要
以模式植物拟南芥为材料,通过PCR和RT-PCR在DNA和RNA水平上筛选鉴定了DTX31基因对应的T-DNA插入突变体,对其表型变化进行了观察.通过半定量RT-PCR分析检测了DTX31基因在拟南芥不同器官及环境胁迫响应中的表达情况,结果发现:DTX31基因在根中表达最高,而在茎、叶、叶柄、花中的表达则较弱;盐和赤霉素使DTX31基因的表达迅速升高,盐胁迫2 h后的表达量达到最高峰,GA处理1 h时就达到最高峰,热激使DTX31基因的表达变化不明显.因此,推测该基因可能是盐和GA信号传导通路中的一个重要调控因子.
The homozygous T-DNA mutants of the DTX31 gene in Arabidopsis were identified at DNA and RNA levels,and its phenotypic changes were observed. The Semi-quantitative RT-PCR was used for the analysis of the expression levels of DTX31 gene in the different tissues and under environmental stresses. It was found that the DTX31 gene was highly expressed in roots, and less in stems, leafstalks, leaves, and flowers. The salt and gibberellic acid （GA） induced a fast increase in mRNA level of the DTX31 gene. The expression of the DTX31 gene increases to the maximum with the salt for 2 h,and with the GA for 1 h. The heat shock at 37℃ had no effect on the expression of the DTX31 gene in the experiments. These results indicated that the DTX31 gene was likely to be an important regulation factor in salt and GA signaling pathways.
出处
《西北植物学报》
CAS
CSCD
北大核心
2008年第4期675-679,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30600368,30770200)
作者简介
郭新红（1975-），女（汉族），博士，副教授，从事植物分子生物学研究。E-mail：xinhong@hnu．cn
通讯作者：刘选明，教授，博士生导师，从事植物生物化学与分子生物学研究。E-mail：sw_xml@hnu．cn
