摘要
目的探讨大鼠慢性缺氧性肺动脉高压(HPH)形成过程中MAPK组织蛋白1(Morg1)在肺内的动态表达和意义。方法40只成年雄性SD大鼠随机分为对照组(C组)和缺氧3d,7d,14d和21d组(H3,H7,H14和H21组),每组8只,常压缺氧复制HPH大鼠模型。测各组大鼠平均肺动脉压(mPAP)、右室肥大指数(RVHI)、血管形态学指标;western-blot检测Morg1蛋白质表达。结果①H7组大鼠mPAP开始上升,与C组比较,差异有显著性(P<0.05),H14组达高水平并维持于此水平。缺氧14d后出现肺血管重塑,RVHI改变。②Morg1蛋白在C组呈弱阳性表达(0.085±0.007),H3,H7组表达升高(0.102±0.003,0.124±0.010),H14组达高峰(0.146±0.006,与C组比较,P<0.05)。结论Morg1蛋白含量在HPH大鼠中呈动态表达,可能在其发病机制中发挥作用。
Objective To investigate the dynamic expression of Morgl in pulmonary arteries of rats with hvpoxia-induced puhnonary hypertension. Methods Male SD rats （n =40） were randomly divided into 5 groups and exposed to hypoxia for 0,3,7,14 or 21d （ C, H3 , H7 , H14, H21 group ） respectively. Mean pulmonary arterial pressure （ mPAP）, vessel morphometry and right ventricle hypertrophy index（RVHI） were easured. Western- blur was used to determine, the expression of protein. Results （1）The level of mPAP was higher in H7 group than in C group,and the difference has a statistical significance（ P 〈0.05） ,reaching its peak after 14 days of hypoxia,theu remained on the high level. Puhuonary artery remodeling index and RVHI became evident after 14 days of hypoxia;（2）Morgl protein was barely positive in C group （ 0. 085 ± 0. 007 ）, then increased in H3 and H7 group （ 0. 102 ± 0. 003,0. 124 ± 0. 010 ） reaching its peak in H14 group（0. 146 ± 0. 006 compared with C group, P 〈 0.05 ）. Conclusion Morgl may involved in the pathogenesis of hypoxia-induced pulmonary hypertension in rats.
出处
《潍坊医学院学报》
2008年第1期66-67,共2页
Acta Academiae Medicinae Weifang
基金
国家自然科学基金资助项目(30270581
30570815)
中国博士后科学基金(2003033436)
教育部科学技术重点项目(03091)
湖南省自然科学基金(05JJ30073)
作者简介
聂会（1983年1月～），女（汉族），山东省潍坊市人，在读硕士研究生。主要研究方向：肺动脉高压
[通讯作者]戴爱国，E-mail：daiaiguo2003@163．com。
