摘要
试验采用批次培养法研究了体外条件下添加大豆油对山羊瘤胃培养液中共轭亚油酸及氢化中间产物累积规律的影响,大豆油的添加量为0和100 mg/瓶。结果表明大豆油组cis-9,trans-11 CLA含量始终显著高于对照组(P<0.05),且在培养8 h时含量达到最高,为6.05 mg/g干物质;大豆油组trans-10,cis-12 CLA含量显著高于对照组(P<0.05),其含量随培养时间的延长而逐渐升高;在培养16 h时,大豆油组总共轭亚油酸含量达到最大,为11.61 mg/g干物质;大豆油组的trans-10C18∶1和trans-11C18∶1含量显著高于对照组(P<0.05),在培养过程中,二者含量始终增加。大豆油组总反式C18∶1脂肪酸含量随着培养时间的延长而逐渐增加,而总顺式C18∶1脂肪酸含量随着培养时间的延长而逐渐下降。
This study was conducted to investigate the effect of addition of soybean oil in dietary on concentrations of conjugated linoleic acid and intermediates of ruminal biohydrogenation of mature goat in vitro by batch culture. The addition level of these soybean oil were 0（control group ） and 100 mg/flask, respectively. Results indicated that the cis-9, trans-11 CLA content of soybean oil supplementation treatment was significantly（P〈0.05）higher than that of the control all the time, and which reached its maximum after 8 hours＇ incubation （6.05 mg/g DM）. And the trans-10, cis-12 CLA content of soybean oil supplementation treatment was also significantly（P〈0.05）higher than that of the control all the time, and which increased gradually, with the extension of incubating time. The total CLA content of soybean oil supplementation treatment reached its maximum after 16 hours＇ incubation （11.61 mg/g DM）. During in vitro study, that trans-11 C18 ： 1 and trans-10 C18 ： 1 were accumulated with treatment of soybean oil, and the treatment was abstractly higher than the control （P〈0.05）. The total trans-C18 ： 1 content of soybean oil supplementation treatment increased gradually, with the extension of incubating time, while the total cis- C18 ： 1 content of soybean oil supplementation treatment decreased gradually.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第4期449-454,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
2002年教育部跨世纪人才基金
“十五”国家西部开发科技行动基金(2002BA901A46)
作者简介
侯俊财（1975-），男，黑龙江省集贤人，讲师，博士，主要从事反刍动物营养和原料奶质量控制研究，Tel；0451-55190459，Fax：045155190577，E-mail：houjuncai88@126．com
通讯作者：霍贵成，教授，主要从事动物营养与乳品科学研究，Tel：0451-55191807，E-mail：gchuo@0451．com
