摘要
【目的】探索离体条件下铁皮石斛体细胞胚发生过程,为铁皮石斛的快速繁殖生产和种质资源保存提供技术依据。【方法】取继代培养30 d的铁皮石斛类原球茎作接种材料,以N6为基本培养基,加入植物激素,并以真菌提取物作诱导子,对类原球茎和愈伤组织进行诱导培养。【结果】显微观察结果表明铁皮石斛体细胞胚的形成可由愈伤组织的表皮细胞或内部细胞产生。【结论】铁皮石斛通过类原球茎和愈伤组织的诱导培养能获得大量的丛芽。
Objective To observe the somatic embryogenesis in in-vitro cultured Dendrobium candidum wall.Ex Lindl.（DCWL）,and to supply evidence for its rapid propagation and germplasm preservation.Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants,N6 was used as the basic culture with phytohormone added,and fungal extracts as the elicitor.Protocorm-like bodies and callus of DCWL were used for induction culture.Results Somatic embryogenesis in in-vitro cultured DCWL derived from the epithelial cells or inner cells of callus of DCWL.Conclusion A large amount of buds can be obtained by the induction and culture of protocorm-like bodies and callus of DCWL.
出处
《广州中医药大学学报》
CAS
2008年第1期74-76,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省自然科学基金资助课题(编号:20000189)
关键词
铁皮石斛
中药培育
组织培养
DENDROBIUM CANDIDUM WALL.EX LINDL.
MEDICINAL HERB ANIMAL RAISING
TISSUE CULTURE
作者简介
潘超美（1956-），女，教授。
