摘要
目的建立小鼠事件相关电位的模型、测定方法及其参考值，探讨具有认知功能改变的血管性痴呆小鼠P300电位的变化特征。方法选择雄性昆明小鼠48只，随机分为正常组、假手术组和模型组。应用双侧颈总动脉线结反复缺血一再灌注法制备血管性痴呆小鼠模型，采用跳台试验和水迷宫试验观测其行为学改变，测定模型组N2、P3的潜伏期及波幅，并与正常组、假手术组进行比较。结果模型组小鼠学习、记忆成绩较假手术组差（P〈0．05），N2、P3潜伏期明显延长（P〈0．01）。结论血管性痴呆小鼠P300电位各波潜伏期显著延长，同时学习、记忆成绩下降。非麻醉状态下经头皮记录小鼠事件相关电位P300可为动物实验研究提供客观、无创、定量和有价值的电生理检测手段。
Objective To built up the ERP model, measure mode and P300 potential reference standard in mice with vascular dementia （VD） , and characterize the P300 potential in mice with VD. Methods Forty- eight mice were randomly divided into a normal group, sham operation group and a VD group. The mice in the Vd group were subject to repetitive isehemia and reperfusion by using the ligation of bilateral common carotid arteries so as to establish the VD model. The behavioral abnormalities were investigated by step-down test and water maze test. The N2 and P3 components of P300 potentials were also recorded. Results It was shown that the learning and memory abilities as reflected by the step down test and water maze test scores were decrease in mice in the VD group when compared with those in the normal group and sham operation group （P 〈 0.05 ）. The N2 and P3 lateneies significantly prolonged （ P 〈 0.01 ） and P3 amplitudes decreased （ P 〈 0.05 ） in VD group as well. Conclusions In VD mice, there is a significant prolongation of the P300 potential latency and a significant decrease of learning and memory abilities. Recordings of P300 from unanesthetized mice could be an objective, non-invasive, quantitative and valuable eleetrophysiologieal method for studying the cognitive function of VD mice.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2008年第3期165-167,共3页
Chinese Journal of Physical Medicine and Rehabilitation
作者简介
通讯作者：吕佩源，Email：ukpeiyuanlu@yahoo．com
