摘要
以生长旺盛的野生旋覆花根茎为外植体,以附加不同浓度的BA、IAA、NAA、2,4-D、GA的MS、1/2 MS或1/3 MS为培养基,进行了旋覆花根茎愈伤组织诱导培养、愈伤组织分化培养、试管苗生根培养和试管苗移栽与扦插的研究。结果表明:MS+2,4-D 1.2 mg.L-1是诱导根茎愈伤组织的理想培养基。MS+Ag(N03)20.4 mg.L-1+BA 0.5 mg.L-1+NAA 0.1 mg.L-1是根茎颗粒状愈伤组织和不定芽分化培养的理想培养基;1/3MS+NAA0.6 mg.L-1是试管苗生根培养和生根增殖培养的理想培养基;炉灰渣是试管苗移栽、扦插的理想基质;移植后旋覆花试管苗生长非常旺盛。
The rhizomes explants from Inula japonica were cultured on the MS, 1/2MS or 1/3MS medium supplemented with BA, IAA, NAA, 2, 4-D, GA of different concentrations to study the callus induction, bud differentiation, rooting, and transplantation of seedings. The results showed that the medium MS ＋2, 4-D 1. 2 mg· L^-1 was the best for inducing callus, medium MS＋Ag （NO3） 20.4 mg·L^-1 ＋ BA 0. 5 mg·L^-1 ＋ NAA 0. 1 mg·L^-1 was the most suitable for bud differentiation, medium 1/3MS ＋ NAA0. 6 mg·L^-lwas the optimum for rooting. The ideal transplantation matrix for tube plantlets was the furnace ashes. The transplanted plantlets in the field grew vigorously.
出处
《农业与技术》
2008年第1期36-40,共5页
Agriculture and Technology
