摘要
目的建立五味子浸膏中五味子醇甲和五味子乙素含量测定方法。方法高效液相色谱法，采用LichrosphereC18柱（4．6mm×250mm，5μm）；流动相为水（A）-甲醇（B）梯度洗脱（0～15min，60—70％B；15—25min，70％～80％B；25～35min，80～75％B；35～45min，75％B）。流速1．0ml·min^-1，柱温30℃，检测波长254nm。结果五味子醇甲和五味子乙素分别在0．1824—1．6416μg（r=1．0003）和0．1896—1．7064μg（r=0．9999）范围内线性关系良好，平均加样回收率分别为98．02％和98．42％。结论该法简便、准确、专属性强，可用于五味子浸膏的质量控制。
Objective To establish a method for the determination of schisandrin and schisandrin B in extract from fruits of Schisandra chinensis. Methods An HPLC method was set up with Lichrosphere C18 （4.6 mm ×250 mm,5μm） column. The Mobile phase was H2O（A） and MeOH（B）. Gradient elution was with 60 -70% B from 0 - 15 min, 70% - 80% B from 15 - 25 min, 80% -75% B from 25 -35 min, and 75% B from 35 - 45 min. The flow rate was 1.0 ml· min^-1, the column temperature was 30℃ and detection wavelength was 254 nm. Results The linear ranges of schizandrin and schisandrin B were within 0. 182 4 - 1. 641 6μg（r = 1. 000 3） and 0. 189 6 - 1. 706 4 μg（r = 0 . 999 9）, the average recoveries were 98.02 % % and 98.42 % respectively. Conclusion This method is easy, sensitive, specific and accurate for the determination of schisandrin and schisandrin B in extract from fruits of Schisandra chinensis.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第3期555-556,共2页
Lishizhen Medicine and Materia Medica Research
基金
江苏省南通市社会发展科技计划项目(No.S40006)
作者简介
宣东平（1963-），男（汉族），江苏南通人，现任江苏省南通市第三人民医院主管药师，主要从事医院药剂工作．
