摘要
从健康牙鲆肠道内分离到一株芽孢杆菌(H-1)和鳗弧菌W-1株进行拮抗实验,表明该菌对鳗弧菌具有较强的拮抗作用(抑菌直径33.05 mm)。应用形态学、生理生化及16S rDNA序列分析方法对该菌进行了综合鉴定,结果显示,菌株H-1与枯草芽孢杆菌标准菌株同源性高达98%,故将其确定为Bacillus subtilis。
A Bacillus strain（H-1） was isolated from the intestinal tract of healthy Paralichthys olivaceus.Antagonistic activity of H-1 was tested against Vibrio anguillarum（W-1）.The results showed that H-1 was found to have strong antagonistic activity against W-1（inhibitory zone diameters=33.05 mm）.Through study on its morphological,physiological and biochemical characteristics as well as 16S rDNA sequence homology comparison,the strain H-1 was identified as Bacillus subtilis,which was almost the closest relative to the standard strain with more than 98% similar in sequence under the phylogenetic tree.
出处
《安徽农业科学》
CAS
北大核心
2008年第6期2321-2322,2326,共3页
Journal of Anhui Agricultural Sciences
基金
科技部社会公益研究专项(2004DIB4JH165)
关键词
芽孢杆菌
抗菌效果
鉴定
Bacillus
Antibiotic effect
Identification
作者简介
黄剑飞（1980-），男，湖北荆门人，硕士研究生，研究方向：动物微生态制剂。
通讯作者，E-mail：lijian@ysfri．ac．cn。
