摘要
目的研究硒对人甲状腺上皮细胞氧化损伤的影响。方法取良性甲状腺腺瘤手术中的腺瘤旁正常甲状腺组织进行细胞培养。加硒（10^-7mol/L）或不加硒后加入不同质量浓度H2O2（0—800μmol／L）刺激单层培养的甲状腺细胞，测定细胞膜GSH—Px活性和MDA含量变化。结果经过H202作用24h的甲状腺细胞，其GSH—Px活力下降、MDA含量升高，并表现为浓度-效应关系。加入10^-7mol／L硒预干预后，各组细胞呈GSH-Px活力升高、MDA含量下降。结论硒对H2O2引起的甲状腺细胞氧化损伤可能有保护作用。
Objective To investigate the influence of selenium （Se） on oxidative damage of human thyrocytes. Methods The human thyroid epithelium cells （TEC） from normal para-adenoma tissues of patients with thyroid adenoma were cultured. H2O2 （0 - 800μmol/L） with different concentrations was added into thyrocytes of monolayer culture with or without Se （ 10^-7 mmol/L）. The activity of GSH-Px and content of MDA were determined. Results When the thyrocytes exposed to H2O2 for 24 hours, the GSH-Px activity declined but MDA content increased with a relation of dose-effect response. Adding Se （10^-7 mmol/L） into cell culture, the activity of GSH-Px was increased, and the content of MDA was descent. Conclusion Selenium could protect thyroid follicular epithelial cell against oxidative damage caused by H2O2.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2008年第2期139-141,共3页
Immunological Journal
关键词
硒
甲状腺上皮细胞
氧化损伤
Selenium
Thyroid follicular epithelial cell
Oxidative damage
作者简介
张方华（1982-），女，山东菏泽人，硕士生，主要从事自身免疫性甲状腺疾病方面的研究。（Tel）0532-82911556；（E-mail）zhangfangh2005@163.com.
通讯作者
