摘要
目的：探讨夏枯草总黄酮的最佳提取工艺及其在不同居群、不同部位间分布情况。方法：应用响应面法确定最佳提取工艺，并对不同居群、不同部位中总黄酮含量进行测定。结果：液固比为25∶1，以35%的乙醇在86 ℃水温下回流提取3. 5 h，可获得夏枯草总黄酮的最佳提取效果。不同居群总黄酮含量变异幅度为2. 16%～10. 29%；叶片中总黄酮含量最高,根中最低；果穗去除种子后总黄酮含量平均高出27. 6%。结论：响应面法对夏枯草总黄酮的提取条件优化合理可行；不同居群间总黄酮含量表现出明显的地理分布差异。
Objective： To study the extraction flavonoids distribution in different parts of Prunella vulgaris. from different populations. Method： The optimal extraction condition was selected by response surface method （RSM）. The amounts of flavonoids were determined by colorimetric method. Result： The optimum extraction condition was using 35% ethanol at 25 times of sawple volume, refluxing at 87 ℃ for 3. 5 h. The amount of flavonoids was 2. 16%-10. 29% in P. vulgaris. The content of flavonoids was the highest in leaf while that in root was the lowest. And the content of flavonoids in spike after removed seeds was 27. 6% higher than the one reserved seeds. Conclusion： The RSM was feasible for optimum extraction condition and the amount of flavonoids showed a signiticant regional distribution pattern in uarious populations of P.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第6期651-653,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30772730)
关键词
夏枯草
居群
总黄酮
响应面
分布特征
Prunella vulgaris
populations
flavonoids
response surface method
distribution
作者简介
[通讯作者]郭巧生，Tel：（025）84396591，E—mail：gqs@njau．edu．cn
