摘要
目的:探讨食管神经递质对食管癌细胞分化的影响及其机制,为防治食管癌与复发提供实验依据。方法:应用EC109细胞系,分为乙酰胆碱(acetylcho-line,Ach)组、Ach加阿托品组、去甲肾上腺素(norepinephrine,NE)组、NE加托拉唑林组、Ach+NE组和对照组,体外培养14d,HE和氧化还原修复酶-1(peroxire-doxin-1,PRX1)免疫组化染色,镜下观察和图像分析,并对结果进行统计学处理。结果:各药物处理组与对照组相比:1)细胞3d长出突起,7d明显长长,14d部分细胞之间相互连结形成网状;2)PRX1免疫组化显色明显加深;3)核质比下降。Ach和NE组3项指标与对照组者相比差异均有统计学意义,P值均<0.05。结论:神经递质Ach和NE对食管癌细胞分化具有诱导作用,其作用可能与其增强DNA修复酶的表达有关,但其确切作用机制尚待进一步研究。该发现为开拓食管癌发病机制和防治研究的新领域提供了基础资料。
OBJECTIVE： To investigate the effect and mechanism of the esophageal nerve transmitters on esophageal cancer ceils differentiation and to provide clues for esophageal cancer prevention and treatment. METHODS： EC109-line ceils were divided into 6 groups： acetylcholine （Ach） group, Ach ＋ Atropine group, norepinephrine （NE） group, NE ＋ tolazoline group, Ach ＋ NE group, and control group. The ceils were cultured for 14 d in vitro, then fixed and stained with HE and peroxiredoxin-1 （PRX1） immunohistochemically staining. The data were analyzed with SPSS 10.0 statistical software. RESULTS： With the comparison of the cell growth and differentiation among the treatment groups and the control group, the chief findings included that.. 1）the ceils grew its processes in 3 days, the processes grew longer in 7 days, and some of them linked up together and became the networks in 14 days; 2） the color stained with PRX1 immunohistochemically increased obviously; 3）the nuclear-cytoplasmic ratio between the cell nuclei and cytoplasm reduced. Three indexes of Ach and NE groups had a significant difference compared with the control group, P〈0.05. CONCLUSIONS： Ach and NE can induce the esophageal cancer cell differentiation, which may be related to the increase of PRX1 expression. These findings provide the basic data for a new research area in esophageal carcinogenesis and prevention.
出处
《中华肿瘤防治杂志》
CAS
2008年第1期4-7,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30025016)
国家科技部863重大项目(2007)
河南省科技攻关项目(20058)
作者简介
吴爱群，女，河南汝南人，教授，主要从事食管贲门癌变机制与防治的研究工作。E-mail：wuaiqun@smmu．edu．cn
【通讯作者简介】王立东，男，河南获嘉人，博士，教授，博士生导师，主要从事食管和贲门癌变机制和防治的研究工作。Tel：86-371-66658335 E-mail；ldwang@zzu．edu．cn
