摘要
目的研究2，2’，4，4’-四溴联苯醚（2，2’，4，4’-tetrabromodiphenyl ethers，PBDE-47）和2，2’，4，4’，5-五氯联苯（2，2’，4，4’，5-hexachlorobiphenyl，PCB153）联合作用的细胞遗传毒性。方法体外培养的SH-SY5Y细胞暴露于2、4、8mol／LPBDE-47或（和）5mol／LPCB153 24h后，采用噻唑盐（MTT）、单细胞凝胶电泳、胞质分裂阻滞以及SDS-KCl沉淀法分别检测细胞活力、DNA损伤、微核和DNA-蛋白交联（DPC）形成情况。结果与单独PBDE-47染毒组比较，各联合染毒组核分裂指数（NDI）明显下降，微核率、微核细胞率和DPC明显增加，Olive尾矩增大，尾部DNA百分率升高，差异均有统计学意义（P〈0．05）。≥4mol／LPBDE-47＋5mol／LPCB153联合染毒细胞存活率明显低于相应剂量的PBDE-47和PCB153单独染毒组，差异有统计学意义（P〈0．05）。≥2mol／L PBDE-47＋5mol／L PCB153联合染毒组微核率、微核细胞率、DPC均明显高于PCB153单独染毒组；≥4mol／L PBDE-47＋5mol／L PCB153联合染毒组细胞NDI低于PCB153单独染毒组；Olive尾矩和尾部DNA百分率仅8μmol／LPBDE-47＋5μmol／L PCB153联合染毒组高于PCB153单独染毒组，差异均有统计学意义（P〈0．05）。析因分析显示，两者在抑制细胞存活、诱导DNA损伤、微核和DPC形成等方面存在交互作用，差异有统计学意义（P〈0．01），表现为协同作用方式。结论一定剂量的PBDE-47与PCB153联合可抑制细胞存活，诱导DNA损伤、微核和DPC形成，呈现细胞遗传毒性，两者联合作用类型为协同作用。
Objective To investigate the cytogenetoxicity of 2,2＇ ,4,4＇-tetrabromodiphenyl ethers （PBDE-47） combined with 2,2＇, 4,4＇, 5-hexachlorobiphenyl （PCB 153）treatment in SH-SY5Y cells. Methods Exponentially growing SH-SY5Y cells were exposed to different concentrations of PBDE-47 or/and PCB153 for 24 h in vitro. Cell viability, DNA damage, chromosome abnormalities, and DNA-protein crosslinks （DPC） were measured using MTT, comet assay, cytokinesis-block micronucleus（CBMN） test, and SDS-KCI assay respec-tively. Results Compared to the each single PBDE-47 groups,the nuclear division index （NDI） was significantly lower （P〈0.05） and the frequencies of micronuclei （MNI）, percentage of DNA in the tail, Olive tail moment and DPC were significantly increased （P〈0.05） in the PBDE-47 combined with PCB153 groups. There was a statistical decrease in cell viability in groups of 4 μmol/L PBDE-47 and above combined with PCB153 than that in contrast to the same dose of PBDE-47 group or PCB153 alone（P〈0.05）. Significant increase was found in MNI frequency and DPC in 2 μmol/L PBDE-47 and above combined with PCB153 than those in the single PCB153 group（P〈0.05）. In the groups of 4 μmol/L PBDE-47 and above combined with PCB153 ,the cell NDI were sig-nificantly lower than that of the single PCB153 group （P〈0.05）. Compared to the single PCB153 group,the percentage of DNA in the tail and Olive tail moment was significantly increased in the 8 μmol/L PBDE-47 combined with 5 μmol/L PCB153. Factorial analysis showed that interactions between PBDE-47 and PCB153 existed in inhibiting cell viability,inducing DNA damage,MNI,and DPC formation （P〈 0.01）,andpossessingsyner-gistic effects. Conclusion Some dose of PBDE-47 combined with PCB153 can inhibit cell viability,induce DNA damage,DPC formation,and chromosome abnormalities. The pattern of the combined effect is synergistic in cytogenotoxicity.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2008年第2期89-93,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金重大项目（45090393）
作者简介
通讯作者：王爱国，E-mail：wangaiguo@mails．tjmu.edu.cn
